FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882752
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT61060.3243124957509008No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48800.25919505064926235No Hit
GTAATACATGGCCGTGTCCGCGGCCGTCACAGAGGTCAATCTCAGGGAGA43560.23136345094839894No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGACCATTAGCAA39720.21096777483173568No Hit
GTCTCGGGTGGCCCCATCAGCAGTGGTAACTGGTGGAGTTGGGTCCGCCA36320.19290910327010674No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33420.177506118702835No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33160.17612516146576926No Hit
ATGTATTACTGTGCGAGAGGGATGACTCCGGCGGGGATATGCTTTGACTC32760.1740006118702835No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31920.1695390577197634No Hit
GATCTATTCTGCATCCATTTTGCAAAGTTCGGTCCCATCAAGGTTCAGTG31550.1675738493439391No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30780.163484091372629No Hit
GAGTTACAGTTCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA29520.15679176014684887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.15323313957441023No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28850.15323313957441023No Hit
CCCCTAAGCTCCTGATCTATTCTGCATCCATTTTGCAAAGTTCGGTCCCA28460.1511617037188116No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG28450.15110858997892448No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG27870.14802799306547013No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26410.1402733870419471No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25680.1363960840301856No Hit
ATTCTGCATCCATTTTGCAAAGTTCGGTCCCATCAAGGTTCAGTGGCAGT25570.135811832891427No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCCTCGGACGTTCGGCCAAGG24600.13065980012237405No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC24270.1289070467060983No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24200.1285352505268883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC24120.12811034060779117No Hit
ACAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATTCTGCATCCATTT23570.12518908491399824No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGA23440.12449860629546536No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA23420.12439237881569107No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC23290.1237019001971582No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23000.12216160174043102No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAAC22710.12062130328370385No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA21900.11631909035284521No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21550.11446010945679516No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA21270.11297292473995513No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21030.11169819498266367No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20600.10941430416751649No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACAGT20570.10925496294785506No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACT20360.10813957441022502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20280.10771466449112789No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20120.10686484465293358No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20010.10628059351417499No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA19940.10590879733496499No Hit
GAATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTCTGTTGATACCAATT19640.10431538513835066No Hit
GTATTACTGTGCGAGAGGGATGACTCCGGCGGGGATATGCTTTGACTCCT19320.10261574546196206No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGA19290.10245640424230062No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19280.10240329050241348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG45400.064.2350853
TCAACGC45950.063.3095054
CAACGCA50900.057.43415
AACGCAG52650.055.6574136
CGCAGAG56100.051.9779478
GCAGAGT63650.045.9255759
ACGCAGA64550.045.062177
GTATCAA80200.037.7303051
TATCAAC88850.033.4707682
GTACATG50700.032.5419041
ACATGGG52250.029.9051883
GTACACG11850.026.7359685
GTAATAC26200.026.398871
TGGCCGT25000.026.2076669
CAGCGCT8150.025.6193079
ATGGCCG25900.025.5749638
TACATGG71450.024.5901242
GTACTAG2351.4061898E-624.526611
TACACGG12700.024.3776446
CATGGGG30700.024.1586114
Produced by FastQC (version 0.11.5)