FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882752
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT56280.29892412808484603No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42490.22568028078047453No Hit
GTAATACATGGCCGTGTCCGCGGCCGTCACAGAGGTCAATCTCAGGGAGA38870.20645310694132843No Hit
GTCTCGGGTGGCCCCATCAGCAGTGGTAACTGGTGGAGTTGGGTCCGCCA33200.17633761642531784No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGACCATTAGCAA33170.1761782752056564No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30440.1616782242164661No Hit
ATGTATTACTGTGCGAGAGGGATGACTCCGGCGGGGATATGCTTTGACTC29340.15583571282888029No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29250.15535768916989598No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29030.15418918689237882No Hit
GATCTATTCTGCATCCATTTTGCAAAGTTCGGTCCCATCAAGGTTCAGTG28640.1521177510367802No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28630.15206463729689307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27170.14431003127337003No Hit
GAGTTACAGTTCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA26970.14324775647562715No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25820.1371396763886056No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25560.13575871915153986No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG24640.13087225508192263No Hit
CCCCTAAGCTCCTGATCTATTCTGCATCCATTTTGCAAAGTTCGGTCCCA24070.12784477190835541No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23510.12487040247467537No Hit
ATTCTGCATCCATTTTGCAAAGTTCGGTCCCATCAAGGTTCAGTGGCAGT22690.12051507580392957No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22690.12051507580392957No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22160.11770004758991096No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCCTCGGACGTTCGGCCAAGG21860.11610663539329662No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGA21760.11557549799442518No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC21450.11392897205792372No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21130.11222933238153512No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20950.11127328506356651No Hit
ACAGAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATTCTGCATCCATTT20700.10994544156638793No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20540.10909562172819363No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA20150.10702418587259502No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACT19940.10590879733496499No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAAC19740.1048465225372221No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19370.10288131416139779No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19060.10123478822489632No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA18920.10049119586647631No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG42750.059.4562953
TCAACGC42750.059.4562954
CAACGCA51600.049.5379375
AACGCAG53250.048.2733966
CGCAGAG53500.047.240298
ACGCAGA60600.041.705547
GCAGAGT61300.041.3379759
GTATCAA74600.034.7428361
GTACATG49950.030.2681141
GAATACG4050.030.2176639
TATCAAC85700.029.9948462
ACATGGG50050.029.0607323
ATGGCCG23900.028.6210088
GTAATAC24450.028.2679251
TGGCCGT24150.027.7225279
CAGCGCT9800.026.444999
GGTATCA54700.025.7970661
GTCTCGG27100.025.5037171
TCGGGTG26600.025.1744824
TACATGG66750.025.0262622
Produced by FastQC (version 0.11.5)