FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192963
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATGGCCGTGTCCTCAGAGGTCAGGCTGCGCAGCTCCATGTAGA43710.3663986225893008No Hit
ATTTATTACTGTGCGAGAGGGACCATAAATTATTACTATGATAGCAGTGC36140.30294317594091347No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA26900.22548897157749237No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGATG26420.2214653765456263No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26130.21903445454720724No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGATGG26000.21794473089274355No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC25740.21576528358381608No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24710.20713131924460357No Hit
GTCTACATGGAGCTGCGCAGCCTGACCTCTGAGGACACGGCCATTTATTA24600.20620924538313426No Hit
CTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGA24310.20377832338471522No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23950.20076062711081566No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22350.18734864367126222No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21620.18122942622696597No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20160.16899099133837345No Hit
GGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGATGGGAATAATCAAC19620.16446444692752415No Hit
CTCCCAGGTGCATCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG17750.14878919128254606No Hit
CTCCTGTACTGGAACCAGCAGTGACGTTGGTGGTTTTAATTATGTCTCCT17570.1472803431455963No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17110.14342439790672468No Hit
TGATAGCAGTGCTCCCTCCTCATTATCCGGGGACTACTGGGGCCAGGGAA17000.14250232404525537No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC16980.14233467425226096No Hit
GATTATTACTGCAGCTCATATACAAGTAGCAGCACTGGTTGGGTGTTCGG16890.1415802501837861No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16800.1408258261153112No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16700.13998757715033913No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16570.13889785349587538No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16570.13889785349587538No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16130.1352095580499982No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAAATAATCAGGGAGG16020.1342874841885289No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15390.1290065157092047No Hit
GCCTTGGGCTGACCTACGACGGTCAGCTTCGTCCCTCCGCCGAACACCCA15300.12825209164072984No Hit
CTGCTATCATAGTAATAATTTATGGTCCCTCTCGCACAGTAATAAATGGC15250.1278329671582438No Hit
CTCCTGGACAGTCGATCACCATCTCCTGTACTGGAACCAGCAGTGACGTT15190.12733001777926056No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG15040.12607264433180243No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14960.12540204515982473No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14900.12489909578084148No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14650.12280347336841126No Hit
GGATACACCTTCACCATCTACTATATGCACTGGGTGCGACAGGCCCCTGG14220.11919900281903127No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14030.1176063297855843No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13880.11634895633812616No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13790.11559453226965127No Hit
GTGCGAGAGGGACCATAAATTATTACTATGATAGCAGTGCTCCCTCCTCA13760.11534305758015966No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13720.11500775799417082No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG13660.11450480861518757No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC13520.11333126006422664No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA13460.11282831068524339No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT13310.11157093723778524No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13260.1111518127552992No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13250.11106798785880198No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13130.11006208910083548No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12990.10888854054987457No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG12400.10394287165653923No Hit
ACATGGAGCTGCGCAGCCTGACCTCTGAGGACACGGCCATTTATTACTGT12390.10385904676004203No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC12210.10235019862309225No Hit
TTATTACTGTGCGAGAGGGACCATAAATTATTACTATGATAGCAGTGCTC12080.10126047496862853No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11940.10008692641766762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)