FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470967
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAAAGATCAGGTTGGTCACCTCCAGGTAGG72810.4949805128191183No Hit
GTATATTACTGTGCGATTACCGCAACTCCGAGGGCCCGTATGGACGTCTG57540.3911712499328672No Hit
TCCCTATATTTCATACAGCGAATTACGCACAGAAGTTCCAGGCCAGAGTC54280.3690089580527639No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT39370.2676470648219844No Hit
ATTTCATACAGCGAATTACGCACAGAAGTTCCAGGCCAGAGTCACCATAA34650.23555932933913543No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC30100.20462729619359238No Hit
GCCGTATATTACTGTGCGATTACCGCAACTCCGAGGGCCCGTATGGACGT29470.2003443992965172No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28470.19354615025354072No Hit
ATATACGGCCGTGTCCTCAAAGATCAGGTTGGTCACCTCCAGGTAGGCTG27980.19021500822248222No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27610.1876996560765809No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA27260.18532026891153913No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26830.18239702182305925No Hit
ATATAGGGATGGTCCCTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGT26630.18103737201446396No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24940.16954833113183368No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24230.1647215743113204No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23320.15853516768221176No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22760.15472814821814493No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22000.1495614789454828No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21940.1491535840029042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.1412676151130515No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGAGTCCCATCAAGGTTCAGTG20300.13800445557242277No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20280.13786849059156325No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19590.13317769875190946No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19360.13161410147202487No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC19160.13025445166342958No Hit
GTATGAAATATAGGGATGGTCCCTCCCATCCACTCAAGCCCTTGTCCAGG19030.12937067928784263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18340.12467988744818885No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG18210.1237961150726019No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC17690.12026102557025412No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT17390.11822155085736118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17210.1169978660296254No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA17110.11631804112532775No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCCGCAG17070.1160461111636087No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17000.11557023373060034No Hit
ATATTACTGTGCGATTACCGCAACTCCGAGGGCCCGTATGGACGTCTGGG16210.11019961698664892No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15940.10836408974504526No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG15850.10775224733117739No Hit
ACCTGGAGGTGACCAACCTGATCTTTGAGGACACGGCCGTATATTACTGT15200.10333338545324267No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15110.10272154303937477No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC15090.10258557805851524No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15060.10238163058722595No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTT14990.1019057531542176No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC14800.10061408583605207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)