FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071805
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATACTTCACCCCGGACGTTTGGCCAGGGGA23610.22028260737727476No Hit
GTAATAAAGGGCCGTGTCGTCAGATCTCAGGCCGCTCAGCTCCATGTAGG22600.21085925144965736No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20810.19415845233041457No Hit
GTCTGGGACTGAGGTGAAGAAACCTGGATCCTCGGTGAAGGTCTCCTGCA17290.16131665741436174No Hit
GTATGATACTTCACCCCGGACGTTTGGCCAGGGGACCAAGCTGCAGATCA15310.14284314777408205No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGGTGATGGTGAGAGT14950.13948432783948572No Hit
CTTTATTACTGTGCGAGAGAGGATCCCCAGAGAGGTTTACAGATTTATGA13970.13034087357308466No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13660.12744855640718228No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12100.11289367002393158No Hit
GCCTACATGGAGCTGAGCGGCCTGAGATCTGACGACACGGCCCTTTATTA11930.11130756061037221No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC11320.10561622683230626No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10790.10067129748415056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13850.058.2995264
ATCAACG14400.058.075413
GTATCAA15050.057.9975661
CAACGCA15750.051.7315645
AACGCAG16300.050.367126
TATCAAC17050.048.626192
ACGCAGA17750.045.851187
CGCAGAG19650.041.4177368
GGGCCGT12550.029.8435429
GGACCGA1504.604513E-528.8064586
GAATACG3800.028.4314089
GTACATG26450.028.091341
TCTACAC9800.026.4833643
GCAGAGT33150.026.0728459
TAAAGGG15100.025.785445
AAGGGCC16350.023.7873697
TTACACC5350.022.908254
AAAGGGC17300.022.062756
GTATTAC20400.021.9241331
GTGCTAG5650.021.705081
Produced by FastQC (version 0.11.5)