FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129512
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCAGATGTCAGGCTGCTCAGCTCCATGTTGG36360.3219089305824108No Hit
GTATATTACTGTGCGATACTAGATCGTCGGCGGGATAGTCGCGGTTTACT33530.29685386255303176No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25060.22186572608347677No Hit
TCTATGATGCGTCCAACAGGGCCACTGGCATCCCAGCCAGATTCAGTGGC22390.19822719900275515No Hit
CCCCTATTATTCGTACACCAAAATATGCACAGAAGTTCCAGGGCAGAGTC22210.1966335904355155No Hit
ATACTAGATCGTCGGCGGGATAGTCGCGGTTTACTTGACATCTGGGGCCA21400.18946235188293706No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20610.1824681809489408No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20090.177864422865804No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT20020.17724468620076633No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17880.15829845101247264No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC17780.15741311291956173No Hit
GCCGTATATTACTGTGCGATACTAGATCGTCGGCGGGATAGTCGCGGTTT17320.15334055769217148No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16560.14661198818604848No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16030.1419196962936206No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15930.1410343582007097No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15000.13280071393663812No Hit
TAATAGGGGTGATCCCTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGT14840.13138417298798066No Hit
ATATACGGCCGTGTCGTCAGATGTCAGGCTGCTCAGCTCCATGTTGGCTG14750.1305873687043608No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14630.12952496299286773No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14180.12554094157476858No Hit
ATCTAGTATCGCACAGTAATATACGGCCGTGTCGTCAGATGTCAGGCTGC13690.12120278491950505No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA13420.11881237206864557No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12890.1141200801762177No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12680.11226087018110477No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11910.10544376686569067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11650.10314188782412227No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT11640.10305335401483119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20200.071.06651
TCAACGC22100.064.209874
ATCAACG22400.063.6714823
CAACGCA23300.061.2120675
TATCAAC24200.058.9355852
AACGCAG24400.058.4059336
CGCAGAG29250.048.7215278
TATACGG12800.045.5829935
ACGCAGA31450.045.5422027
ATACGGC13350.043.131086
TACGGCC13600.042.3382267
GTAATAT17100.037.545431
GTACACG11100.035.6917275
ATATACG17150.035.2811664
TACACGG11500.035.048696
AATATAC18550.034.1716963
GTACATG30950.032.6310161
TAATATA20300.032.2903822
TACATGG30900.031.9366872
TATGTAC13000.031.583432
Produced by FastQC (version 0.11.5)