FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129512
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCAGATGTCAGGCTGCTCAGCTCCATGTTGG35310.3126128806068461No Hit
GTATATTACTGTGCGATACTAGATCGTCGGCGGGATAGTCGCGGTTTACT25700.22753188987810666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24240.2146059537216072No Hit
TCTATGATGCGTCCAACAGGGCCACTGGCATCCCAGCCAGATTCAGTGGC18520.16396461480710253No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18040.15971499196113012No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17970.15909525529609247No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT17750.15714751149168846No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17200.15227815198067837No Hit
ATACTAGATCGTCGGCGGGATAGTCGCGGTTTACTTGACATCTGGGGCCA16860.14926800246478125No Hit
CCCCTATTATTCGTACACCAAAATATGCACAGAAGTTCCAGGGCAGAGTC15740.13935221582417895No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15720.13917514820559676No Hit
TAATAGGGGTGATCCCTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGT14710.13023323346719645No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14540.1287281587092479No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC14370.12722308395129933No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14120.12500973871902202No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13970.12368173157965563No Hit
ATATACGGCCGTGTCGTCAGATGTCAGGCTGCTCAGCTCCATGTTGGCTG13680.12111425111021397No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13230.11713022969211481No Hit
GCCGTATATTACTGTGCGATACTAGATCGTCGGCGGGATAGTCGCGGTTT13130.11624489159920391No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12960.11473981684125534No Hit
ATCTAGTATCGCACAGTAATATACGGCCGTGTCGTCAGATGTCAGGCTGC12710.11252647160897802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14450.057.7980461
TCAACGC14350.056.6277584
ATCAACG14750.054.6045463
CAACGCA16050.050.18625
AACGCAG17100.047.531496
TATCAAC17500.045.6129072
CGCAGAG19450.040.309378
TATACGG17350.040.2081035
ATACGGC17450.039.570816
TACGGCC18100.037.752367
AATATAC20750.034.65653
ACGCAGA22850.034.3114747
CAGCGCT5550.033.6960879
ATATACG20900.033.3755384
GTAATAT21800.033.0268021
GCGTTAC900.003771870631.9680848
GTACACG8850.031.6929515
TACATGG27700.031.6725392
TACACGG8800.031.0598966
GTACATG28550.030.7664011
Produced by FastQC (version 0.11.5)