FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849187
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGTAGAGTAGGCAGTAAAAGTCAGCCTCGTCCTCAGCCAGTACGCC30110.3545744341352376No Hit
CTACTATGGTGGTGCCCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC26590.313123022373164No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTG26470.3117099060630933No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGTCTGCTCATGTGCAGAAACA25920.3052331229752693No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG23930.28179894416659695No Hit
GTCCAGGAGTGTCTCTTGTTTGTACTGTAAATCAGTGTCCTGGGTGCTTG22460.264488269368231No Hit
ATATATTACTGTGTGAAAGAGGAGGAGTGTAATAGTGTCACCTGCGACGC21790.25659836997033636No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21500.25318333888766553No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT21350.2514169435000771No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA16050.18900430647195496No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14720.17334226736867145No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14570.1715758719810831No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14440.1700449959785065No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14220.1674542827433769No Hit
ATATATGGCCGTGTCCTCGGCTCTCAGTCTGCTCATGTGCAGAAACAGTG14060.16557012766328266No Hit
GCCATATATTACTGTGTGAAAGAGGAGGAGTGTAATAGTGTCACCTGCGA14030.16521684858576496No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13960.16439253073822374No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13610.16027094150051754No Hit
CTATTACACTCCTCCTCTTTCACACAGTAATATATGGCCGTGTCCTCGGC12670.1492015304049638No Hit
ATATTACTGTGTGAAAGAGGAGGAGTGTAATAGTGTCACCTGCGACGCAA12200.14366682485718693No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTGACTCTCACCTGTGCTTCCA12160.14319578608716338No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12100.142489227932128No Hit
CCACCATAGTAGAGTAGGCAGTAAAAGTCAGCCTCGTCCTCAGCCAGTAC12060.14201818916210446No Hit
ATAGTAGAGTAGGCAGTAAAAGTCAGCCTCGTCCTCAGCCAGTACGCCTG11930.14048731315952787No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11880.13989851469699843No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11570.13624796422931582No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11490.13530588668926868No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11350.1336572509941862No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11300.13306845253165675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.13247965406912732No Hit
GTGTAATAGTGTCACCTGCGACGCAAGTTGGTGGGGCCAGGGAACCCTGG11230.1322441346841155No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11080.13047773929652715No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGTCTGCTCATGTGCAGAAA11070.13035997960402126No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGGCACCACCA10970.12918238267896234No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10950.12894686329395058No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10920.12859358421643288No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10910.128475824523927No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10840.12765150667638578No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10760.12670942913633865No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10630.12517855313376205No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10530.12400095620870315No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10470.12329439805366782No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10320.12152800266607945No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10240.12058592512603232No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT10160.11964384758598519No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10100.11893728943094983No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10070.11858401035343216No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9630.11340258388317297No Hit
GTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGG9600.11304930480565528No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9540.11234274665061995No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC9480.1116361884955846No Hit
CTCTACTATGGTGGTGCCCGGGTGTTCGGCGGAGGGACCAAGCTGACCGT9400.11069411095553747No Hit
GTACAAACAAGAGACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTC9400.11069411095553747No Hit
GATTTACAGTACAAACAAGAGACACTCCTGGACCCCTGCCCGGTTCTCAG9360.11022307218551392No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9270.1091632349529609No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9230.10869219618293734No Hit
TCCTCAGCCAGTACGCCTGACAGTGTCAGGGCAGCCTTGCCCCCAAGGAG9050.10657252171783128No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8800.10362852940518402No Hit
GTAGAGTAGGCAGTAAAAGTCAGCCTCGTCCTCAGCCAGTACGCCTGACA8790.10351076971267811No Hit
CCTTTAACACCAATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG8720.10268645186513689No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAT8520.10033125801501908No Hit
GTAAATCAGTGTCCTGGGTGCTTGTCCAGGTTTCTGCTGGACCCAGCTTG8510.10021349832251318No Hit
ACTATGGTGGTGCCCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTT8500.10009573863000729No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.073.924871
GTATCAA19050.067.34731
ATCAACG19950.063.5865063
TCAACGC20250.062.644484
TATCAAC21000.060.407182
CAACGCA21300.059.5563775
AACGCAG22750.055.7571876
TCGATCA400.006219829453.9970869
CGCAGAG23800.053.2439588
GCAGAGT26050.048.642299
ACGCAGA27600.045.9376147
GTACACG5800.041.0091865
ACGGGGT6100.038.9487159
GAATACG3950.038.2764139
GTATGTA7950.037.1716581
GTGCTAG4500.036.839231
TACACGG6550.036.311346
CAGCGCT3250.035.444249
CTAGAGA4750.034.9003224
TTACGGG1051.9183548E-434.322263
Produced by FastQC (version 0.11.5)