FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642640
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC14450.22485372837047182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.2030685920577617No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12760.19855595667870035No Hit
ACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATAATTAGCACTGC12500.19451014564919705No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA12140.18890825345450019No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT12050.18750778040582597No Hit
GTCTTTATATATCAGCAACACAGGGGCCTGGCCTGACTTGTGCTGGTACC11980.18641852359019045No Hit
GACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATAATTAGCACTG11480.1786381177642226No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCCCTGATGGTCAACGTGG10600.1649446035105191No Hit
CTCCAGGACAGACGGCCAGGATCATCTGCTCTGGAGATGTATTGTCACAG10500.16338852234532553No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC10170.1582534545001867No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT9710.15109548114029628No Hit
TATAAAGACAGTCAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC9160.1425370347317316No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCTCCAGGACAGACG9060.14098095356653803No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8390.13055520975974105No Hit
ATATAAAGACAGTCAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT8240.1282210880119507No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA8170.1271318311963152No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7780.12106311465206025No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC7640.11888460102078924No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGCTAATTATGTCTTCGGAAC7610.11841777667123117No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7410.11530561434084402No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG7250.11281588447653429No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7000.10892568156355036No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6930.10783642474791486No Hit
CCCCTGATGGTCAACGTGGCTATTGTCCCTGAGCTGGAGCCAGAGAATCG6660.1036350056018922No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6520.10145649197062119No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6430.10005601892194697No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.074.01061
GGTATCA4550.068.150641
TCAACGC16650.065.827664
ATCAACG17050.064.283323
CAACGCA17100.064.095355
AACGCAG18450.059.3961946
TATCAAC18550.059.0852052
ACGCAGA19750.055.439057
CGCAGAG20150.053.5985078
GCAGAGT27650.039.3173759
GTACATG17700.032.59341
CAGAGTA22550.031.6075889
CATGGGG12450.031.2754444
GAATACG3000.031.1978579
GTGTAGA3550.030.4702361
GTATAGG2202.3823304E-829.5007271
GTCTAGA1255.315778E-428.8451581
TAGACTC1504.5621695E-528.8429075
GTGCTAG3750.026.9221461
AGTGTAC1658.8188965E-526.182068
Produced by FastQC (version 0.11.5)