FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642640
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12250.19061994273621313No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA12110.1884414291049421No Hit
GTCTTTATATATCAGCAACACAGGGGCCTGGCCTGACTTGTGCTGGTACC11870.18470683430847754No Hit
GACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATAATTAGCACTG11820.18392879372588075No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT11650.18128345574505167No Hit
ACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATAATTAGCACTGC10790.1679011577243869No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC10590.16478899539399974No Hit
CTCCAGGACAGACGGCCAGGATCATCTGCTCTGGAGATGTATTGTCACAG10410.1619880492966513No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT9990.1554525084028383No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCCCTGATGGTCAACGTGG9730.15140669737333498No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA9020.1403585211004606No Hit
TATAAAGACAGTCAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC8690.1352234532553218No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCTCCAGGACAGACG8270.12868791236150876No Hit
ATATAAAGACAGTCAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT7920.12324162828333127No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7840.1219967633511764No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7840.1219967633511764No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC7830.12184115523465704No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7410.11530561434084402No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC7280.11328270882609236No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7230.11250466824349557No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG7040.10954811402962777No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6970.10845885721399227No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6750.10503547865056641No Hit
CCCCTGATGGTCAACGTGGCTATTGTCCCTGAGCTGGAGCCAGAGAATCG6640.10332378936885349No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGCTAATTATGTCTTCGGAAC6630.10316818125233411No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6620.10301257313581477No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6590.1025457487862567No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG451.2256773E-464.099245
ACGTACG400.00617408854.096411
TCAACGC9000.051.2673954
GTATCAA9250.050.684921
ATCAACG9150.050.4269453
CAACGCA9600.047.3232735
GGTATCA5200.044.3867951
AACGCAG10600.042.822056
TATCAAC11000.042.6014562
ACGCAGA11450.039.6462147
CGCAGAG11650.038.9655888
GAATACG2450.038.2305039
GTGCTAG3650.031.6179921
TCTACAC7250.030.826733
GTACATG17250.030.1058251
TAGGGGG1504.5602654E-528.8446645
CCTACGT1000.00624143128.837912
TAGCTCG1100.00996708826.1999249
CTAGAGA4450.025.9217154
ACATGGG19500.025.5104563
Produced by FastQC (version 0.11.5)