FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004420
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC26560.26443121403396985No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20330.2024053682722367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.17811274168176658No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15910.15839987256327034No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13520.1346050456980148No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13250.13191692718185621No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG12800.12743672965492525No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12690.12634157025945322No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10600.10553354174548495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.080.151891
GTATCAA24450.078.775051
ATCAACG31250.060.1735233
TCAACGC31300.060.0773964
CAACGCA32300.058.434655
AACGCAG34900.054.2391246
TATCAAC35250.053.5497062
CGCAGAG38350.048.984368
GCAGAGT43800.043.0536089
ACGCAGA45500.041.286827
CAGCGCT8650.039.9399459
GCAGCGC8950.039.4053658
GGCAGCG9150.037.757437
GTACATG31300.028.578121
TACATGG30400.027.7285542
CAGAGTA44650.027.7261359
GTACACG14650.026.553875
CTTCGCC1907.5709177E-626.5171459
ACATGGG32100.026.0356183
TACACGG15800.024.5990896
Produced by FastQC (version 0.11.5)