FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937561
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16850.17972163944532674No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12290.13108480408208106No Hit
GTCTCTGGTGACTCCATCAGCAGTACTGGTTACTCCTGGAGCTGGATCCG9990.10655306694711064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11950.059.5999531
TCAACGC12000.056.3299644
CAACGCA13350.051.172335
ATCAACG13250.050.4730953
TATCAAC14250.047.4357572
AACGCAG14700.046.443046
CGCAGAG16900.040.4707078
ACGCAGA18150.037.2868047
GTACATG24900.034.6705361
GCAGAGT20350.033.2557959
TATAACG1102.5555943E-432.686632
ACATGGG24450.032.6465263
TACATGG25850.031.4348032
TAATACG1204.2566046E-429.9627484
CATGGGG16600.029.8905474
ATACGCC1050.00804654927.376956
CAGCGCT4800.026.9982229
GTATTAA4150.026.0029011
TCCTATA3358.367351E-1125.7590182
GTACACG5250.024.655065
Produced by FastQC (version 0.11.5)