FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727127
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC67190.38902755848296044No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT62380.3611778404251685No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC54150.31352645173169086No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACAC49510.286661027243509No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA49460.28637152913480013No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACA49130.2844608416173217No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT48720.2820869571259091No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA46400.2686542448818182No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA43540.25209495306367163No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC39030.22598222365813286No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT37700.2182815739664773No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT37290.21590768947506467No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC36010.20849653789211794No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC35920.20797544129644202No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35920.20797544129644202No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG33730.19529542413499412No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG33680.19500592602628528No Hit
ATAATAAATAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGGAGATAGA33390.1933268369957739No Hit
GTAAAAATCAGACTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG33130.19182144683048785No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33130.19182144683048785No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA32030.18545248843889303No Hit
GTCTGAGGATGAGTCTGATTTTTACTGTGCAGCATGGGATGACAGTGTCA30700.17775183874723746No Hit
GTGCAGCATGGGATGACAGTGTCAAAGGTTTTGTCTTCGGAACTGGGACC30660.17752024026027038No Hit
ATTTATTATTGTGCGAATGGCGTGGAGGGCCACCATTGTACTGGCACTAC29910.17317776862963755No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT29210.16912479510771355No Hit
GATTTTTACTGTGCAGCATGGGATGACAGTGTCAAAGGTTTTGTCTTCGG29160.1688352969990047No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC28940.16756150532068576No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG27280.15795016811155174No Hit
GCATGGGATGACAGTGTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGT26430.15302870026350118No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC25700.14880202787635188No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG25690.14874412825461011No Hit
GTCATCCCATGCTGCACAGTAAAAATCAGACTCATCCTCAGACTGGAGCC25240.1461386452762304No Hit
ATCCCATGCTGCACAGTAAAAATCAGACTCATCCTCAGACTGGAGCCCAC25190.14584914716752156No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAA25020.14486485359791146No Hit
ATGCTGCACAGTAAAAATCAGACTCATCCTCAGACTGGAGCCCACTGATG24970.14457535548920258No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG24740.14324366418914186No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC24670.14283836683694945No Hit
GGATGACAGTGTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGTCACCG24020.13907489142373433No Hit
CTGTCATCCCATGCTGCACAGTAAAAATCAGACTCATCCTCAGACTGGAG23550.1363536092018711No Hit
CTCCAGAGACGATTCCAAGAACACCCTCTATCTCCAAATGAACAGCCTGA23530.13623780995838755No Hit
GTGCAGGGTCCTGGGCCCAGGCTGTCCTGACGCAGCCACCCTCAGCGTCT23480.1359483118496787No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23340.13513771714529388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.13450082130613442No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG22880.13247433454517243No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG22840.13224273605820533No Hit
CCGTAAACTGGTACCAACAACTCCCAGGAGCGGCCCCCAAACTCCTCATC22030.12755286669712187No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT21810.1262790750188029No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21430.1240788893926156No Hit
TGGGATGACAGTGTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGTCAC21390.1238472909056485No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21290.12326829468823079No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21050.1218787037664283No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG20420.11823102759669672No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20250.11724673402708659No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20150.11666773780966888No Hit
GTCCTGGGCCCAGGCTGTCCTGACGCAGCCACCCTCAGCGTCTGGGACCC20060.11614664121399294No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG20010.1158571431052841No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT19960.11556764499657524No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCG19560.1132516601269044No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTC18880.10931448584846395No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGGCTGTCCTGACGCAGCCAC18860.1091986866049804No Hit
GTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCA18670.10809859379188676No Hit
TCTCTGGAGAGGGTGAATCGGCCCTTCACGGAGTCTGCGTAAAATTTAGT18460.10688270173530956No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA18190.10531941194828175No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18160.10514571308305642No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18050.10450881724389693No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG17630.10207703313074255No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA17460.10109273956113245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.086.324211
GTATCAA34100.075.8344041
TATCAAC40250.064.2212452
TCAACGC40050.064.18614
ATCAACG41050.062.6224943
CAACGCA43500.059.0937655
AACGCAG45400.056.619046
CGCAGAG48350.053.01568
GCAGAGT55400.046.3989949
TACATGG47650.036.114792
ACATGGG48300.035.3326723
GTACATG49200.035.2840271
CAGCGCT7200.035.001429
ACGCAGA78250.032.6658637
GTATGTA15900.032.1652871
GTACACG12950.030.5812785
TATGTAC14750.030.2656352
TACACGG13550.029.2262786
GGATATT10000.028.8128491
ATGGGAG25050.028.7445165
Produced by FastQC (version 0.11.5)