FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727127
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC55220.31972171125806037No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT54440.3152055407622022No Hit
GTCCTGGGCCCAGGCTGTCCTGACGCAGCCACCCTCAGCGTCTGGGACCC50180.2905403019002077No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC46400.2686542448818182No Hit
GTGCAGGGTCCTGGGCCCAGGCTGTCCTGACGCAGCCACCCTCAGCGTCT46350.26836474677310934No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT45600.2640222751424765No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGGCTGTCCTGACGCAGCCAC39650.22957200020612265No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39610.22934040171915557No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37640.21793417623602665No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA36670.21231791292707483No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACAC34710.2009695870656877No Hit
GTCCTGACGCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAC34180.19790090711337383No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC33820.19581652073067007No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACA33540.19419533132190048No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33480.19384793359144983No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC32690.18927386347384995No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT32250.18672628011721198No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31670.18336810205618925No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30840.17856243345162226No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30760.1780992364776881No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA30680.17763603950375392No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG30330.17560955274279194No Hit
ATAATAAATAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGGAGATAGA29940.17335146749486285No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC28180.16316113406831112No Hit
GTCTGAGGATGAGTCTGATTTTTACTGTGCAGCATGGGATGACAGTGTCA27590.15974505638554662No Hit
ATTTATTATTGTGCGAATGGCGTGGAGGGCCACCATTGTACTGGCACTAC27460.1589923613029036No Hit
GTGCAGCATGGGATGACAGTGTCAAAGGTTTTGTCTTCGGAACTGGGACC27190.1574290715158758No Hit
GATTTTTACTGTGCAGCATGGGATGACAGTGTCAAAGGTTTTGTCTTCGG26850.15546048437665558No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT26770.1549972874027214No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG24340.140927679319471No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG23670.13704840466277232No Hit
GCATGGGATGACAGTGTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGT23030.133342828871299No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22820.1321269368147218No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC22680.131316342110337No Hit
CCGTAAACTGGTACCAACAACTCCCAGGAGCGGCCCCCAAACTCCTCATC22350.12940565459285852No Hit
CTCCAGAGACGATTCCAAGAACACCCTCTATCTCCAAATGAACAGCCTGA21430.1240788893926156No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC21280.12321039506648904No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG21250.12303669620126371No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG21000.12158920565771944No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20810.12048911284462578No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20540.11892582305759797No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT20450.11840472646192203No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20400.11811522835321318No Hit
GGATGACAGTGTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGTCACCG19970.11562554461831702No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGGCTGTCCTG19910.11527814688786639No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19700.11406225483128918No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19440.11255686466600312No Hit
GTAAAAATCAGACTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG19310.11180416958336012No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG19220.11128307298768417No Hit
TGGGATGACAGTGTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGTCAC19120.11070407677026646No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAA18800.10885128887452979No Hit
GGCCCAGGCTGTCCTGACGCAGCCACCCTCAGCGTCTGGGACCCCCGGAC18670.10809859379188676No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCG18210.10543521119176529No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18100.1047983153526058No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT17900.10364032291777037No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT17730.10265602934816026No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17710.10254023010467672No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17520.10144013729158306No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGGCTGTCCTGACGCAGCCA17360.10051374334371474No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCC17340.1003979441002312No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)