FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067500
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38880.18805320435308345No Hit
GATATACACTGGTACCAGCAGCTTCCCGGAACAGCCCCCAAACTCCTCAT21950.10616686819830713No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21380.10340991535671101No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20980.10147521160822248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.075.616441
GTATCAA49300.074.62881
ATCAACG57600.062.53
TCAACGC59300.060.829684
TATCAAC66100.054.898642
CAACGCA68550.052.621445
AACGCAG71400.050.5210086
CGCAGAG72100.049.5312048
ACGCAGA83900.042.736597
GCAGAGT83450.042.708219
GTACACG12400.030.1935485
TACACGG13650.027.4285726
GTACATG58500.026.2153851
CAGCGCT8500.025.4117649
TACATGG58800.024.8571412
CAGAGTA82750.024.7975839
ACATGGG58100.024.4130843
ACACGGG16900.022.5798847
CTGTGCG8100.022.2222219
TAGCGCG4256.730261E-1122.023534
Produced by FastQC (version 0.11.5)