FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016079
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTATTGTGTGAGAGTCGTAGTGCCAGCTGCAATTCAGGACTATTT39000.3838284227899602No Hit
ATAATAGACAGCCGTGTCGTCGGCTCTGAGGCTGTTCATCTGGAGATAAA38880.38264741225829885No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA30620.30135452066227136No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC28050.276061211775856No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27380.2694672363074131No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25550.2514568256995765No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24760.24368183969947216No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23190.22823028524356867No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22700.2234078255726179No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22690.22330940802831276No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG20930.205987920230612No Hit
CTATTATTGTGTGAGAGTCGTAGTGCCAGCTGCAATTCAGGACTATTTTT20390.20067337283813563No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20250.19929552721786398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19970.19653983597732066No Hit
ATATTAGTCCTACTGGTAGTTACATAAACTACGCAGAGCCAGTGAGGGGC19750.19437465000260806No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19430.19122528858484428No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17490.1721322849896514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16950.16681773759717503No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16110.15855066387554512No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCACTGGG15470.15225194104001755No Hit
CAATAATAGACAGCCGTGTCGTCGGCTCTGAGGCTGTTCATCTGGAGATA15430.15185827086279707No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15360.15116934805266125No Hit
GATCTATGCTGCATCTAATTTGCAAAGTGGGGTCCCCTCAAGGTTCAGCG15060.14821682172350772No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14960.14723264628045654No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14900.14664214101462583No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG14650.14418170240699787No Hit
CTCCAGAGACAACGCCAAGAACTCACTTTATCTCCAGATGAACAGCCTCA14450.14221335152089554No Hit
GGGTATTAGTACCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCC14420.14191809888798018No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC14400.14172126379936992No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14390.14162284625506483No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT14330.1410323409892341No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13720.1350288707866219No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12890.1268602146092971No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC12880.126761797064992No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA12800.12597445671055105No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12780.1257776216219408No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12770.1256792040776357No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12710.12508869881180498No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12640.12439977600166917No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12550.12351401810292309No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12540.12341560055861799No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT12540.12341560055861799No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12440.12243142511556682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.12144724967251563No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12330.1213488321282105No Hit
GACATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTC12280.12085674440668491No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12100.1190852286091928No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12050.1185931408876672No Hit
CTCCTGTGCAGCCTCCGGATTCATCTTCAGTAGATATGACATGCACTGGG12020.11829788825475185No Hit
TACATAAACTACGCAGAGCCAGTGAGGGGCCGATTCACCATCTCCAGAGA11990.1180026356218365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11920.11731371281170067No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11850.11662479000156484No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11840.11652637245725972No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC11760.11573903210281879No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11630.11445960402685224No Hit
AAACTACGCAGAGCCAGTGAGGGGCCGATTCACCATCTCCAGAGACAACG11510.11327859349519083No Hit
GGCTAAGAATTTCCCGTACACTTTTGGCCAGGGGACCAAACTTGATATCA11490.11308175840658058No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA11260.11081815488756287No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG11150.10973556190020657No Hit
CATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA11120.10944030926729122No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA11040.10865296891285028No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC11000.10825929873562981No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCGGGGTATTAGTAC10980.10806246364701956No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10930.10757037592549398No Hit
ATTAGTACCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA10900.10727512329257863No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10760.10589727767230699No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG10570.10402734433050972No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCGGG10540.10373209169759437No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC10220.10058273027983061No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10220.10058273027983061No Hit
GTTTTAGAAGGTGTCCAATGTGACGTGAATCTGGTGGAATATGGGGGAGG10190.10028747764691526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)