FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016079
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTATTGTGTGAGAGTCGTAGTGCCAGCTGCAATTCAGGACTATTT40440.39800054916989724No Hit
ATAATAGACAGCCGTGTCGTCGGCTCTGAGGCTGTTCATCTGGAGATAAA34450.33904844013113156No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27230.2679909731428364No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26910.26484161172507253No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC26220.25805080116801943No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26100.256869790636358No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23670.23295432737021432No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23380.23010021858536592No Hit
ATATTAGTCCTACTGGTAGTTACATAAACTACGCAGAGCCAGTGAGGGGC20740.20411798688881475No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20670.20342906407867895No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19420.19112687104053916No Hit
CTATTATTGTGTGAGAGTCGTAGTGCCAGCTGCAATTCAGGACTATTTTT19150.188469597344301No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18900.18600915873667304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18630.18335188504043484No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17120.16849083585036204No Hit
CTCCAGAGACAACGCCAAGAACTCACTTTATCTCCAGATGAACAGCCTCA16840.16573514460981872No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16600.16337312354649589No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16560.1629794533692754No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16220.15963325686290142No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16140.15884591650846047No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15980.15727123579957858No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15460.15215352349571243No Hit
CAATAATAGACAGCCGTGTCGTCGGCTCTGAGGCTGTTCATCTGGAGATA14890.1465437234703207No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14480.1425086041538109No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14020.13798139711577545No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCACTGGG13940.1371940567613345No Hit
GATCTATGCTGCATCTAATTTGCAAAGTGGGGTCCCCTCAAGGTTCAGCG13780.1356193760524526No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13240.13030482865997622No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13130.12922223567261992No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG13010.12804122514095853No Hit
GGGTATTAGTACCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCC12710.12508869881180498No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC12690.12489186372319475No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12530.12331718301431287No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12500.12302193038139751No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12280.12085674440668491No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA11940.1175105479003109No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT11900.11711687772309042No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11670.11485327420407271No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11440.112589670685055No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11440.112589670685055No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11380.1119991654192243No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC11300.11121182506478335No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA11160.10983397944451169No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11130.10953872681159635No Hit
GACATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTC11000.10825929873562981No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10940.10766879346979912No Hit
TACATAAACTACGCAGAGCCAGTGAGGGGCCGATTCACCATCTCCAGAGA10930.10757037592549398No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10890.10717670574827351No Hit
CTCCTGTGCAGCCTCCGGATTCATCTTCAGTAGATATGACATGCACTGGG10870.10697987065966329No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10710.10540518995078138No Hit
GGCTAAGAATTTCCCGTACACTTTTGGCCAGGGGACCAAACTTGATATCA10650.10481468468495067No Hit
GTTTTAGAAGGTGTCCAATGTGACGTGAATCTGGTGGAATATGGGGGAGG10590.10422417941911997No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10550.1038305092418995No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10530.10363367415328927No Hit
AAACTACGCAGAGCCAGTGAGGGGCCGATTCACCATCTCCAGAGACAACG10470.10304316888745854No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT10390.10225582853301762No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG10320.1015669057228818No Hit
CATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA10310.10146848817857666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)