FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454176
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT72130.4960197390137095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60990.419412780846335No Hit
ACTATGTACATTGGTATCAGATGAAACCAGGGAAAGCCCCTAAACTCCTG42180.2900611755385868No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40980.28180907950619455No Hit
GAGTTTACTTTCACCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACATA38950.2678492837180644No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35820.246325066566908No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35500.24412450762493673No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG35110.24144257641440925No Hit
GTACATTGGTATCAGATGAAACCAGGGAAAGCCCCTAAACTCCTGATCTA32170.22122494113504831No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31150.21421065950751492No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT29180.20066346852100433No Hit
ATTAATAACTATGTACATTGGTATCAGATGAAACCAGGGAAAGCCCCTAA27330.18794148713773298No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26650.18326529938604405No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA26070.17927678630372113No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25190.17322524921330018No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24840.17081838787051912No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA24160.16614220011883019No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG23560.16201615210263406No Hit
ACATACTGCTGTCAACAATATGAAAATCTCCCTCTCACTTTCGGCGGAGG23170.1593342208921066No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT23100.15885284862355037No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG23030.15837147635499418No Hit
CTATGTACATTGGTATCAGATGAAACCAGGGAAAGCCCCTAAACTCCTGA22910.15754626675175493No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21460.14757498404594768No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGGCATTAATAA21050.14475551790154698No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC20820.14317386616200514No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC20800.14303633122813195No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20750.14269249389344893No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG20580.1415234469555267No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20390.14021686508373127No Hit
GATGAAACCAGGGAAAGCCCCTAAACTCCTGATCTACGATGCATCCCATT20130.1384289109433796No Hit
GCTGTCAACAATATGAAAATCTCCCTCTCACTTTCGGCGGAGGGACCAAG19780.13602204960059855No Hit
GGTATCAGATGAAACCAGGGAAAGCCCCTAAACTCCTGATCTACGATGCA19210.13210230398521225No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC19150.13168969918359263No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18860.12969544264243119No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18700.12859516317144554No Hit
ATACTGCTGTCAACAATATGAAAATCTCCCTCTCACTTTCGGCGGAGGGA18380.1263946042294743No Hit
GCAACATACTGCTGTCAACAATATGAAAATCTCCCTCTCACTTTCGGCGG18380.1263946042294743No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA18140.12474418502299585No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG17490.12027429967211671No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG17420.11979292740356051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.11876141539951146No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG17270.11876141539951146No Hit
TTGCAACATACTGCTGTCAACAATATGAAAATCTCCCTCTCACTTTCGGC17260.11869264793257489No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17230.11848634553176507No Hit
CCCCTAAACTCCTGATCTACGATGCATCCCATTTGGAAACAGGGGTCCCA17020.11704222872609643No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16680.11470413485025197No Hit
ATGTACATTGGTATCAGATGAAACCAGGGAAAGCCCCTAAACTCCTGATC16550.11381015778007614No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16520.11360385537926634No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC16460.11319125057764673No Hit
GTTGACAGCAGTATGTTGCAACATCCTCAGGCTGCAGGCTGCTGATGGTG16410.11284741324296371No Hit
TCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGA15640.10755231828884536No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15590.10720848095416234No Hit
GCCTGAGGATGTTGCAACATACTGCTGTCAACAATATGAAAATCTCCCTC14990.10308243293796625No Hit
TCCTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTCTGTCCCAGAGCC14980.10301366547102965No Hit
AGTCAGGGCATTAATAACTATGTACATTGGTATCAGATGAAACCAGGGAA14930.10266982813634663No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14920.10260106066941002No Hit
AGTAAACTCTGTCCCAGAGCCACTTCCGCTGAACCTTGGTGGGACCCCTG14680.10095064146293158No Hit
ATGTTGCAACATCCTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTCT14600.10040050172743878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)