FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943899
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAGGAGACGGTGACCGTGGCCCCTTGGCCCCAGACGTCCATACCGT20960.2220576565924956No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20960.2220576565924956No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA20890.22131605182334127No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19360.2051066904403967No Hit
GTCTCTGGTGGCTCCATCAATAGTTACTACTGGAGCTGGATCCGGCAGCC19290.20436508567124237No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18870.1999154570563164No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATGGTTACCA18190.19271129644167437No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC16280.1724760805976063No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15180.16082229136803833No Hit
GGTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCC15140.16039851721423584No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG14580.15446567906100123No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14270.1511814293690321No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13730.14546047829269868No Hit
GTGGTAACCATGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT13420.14217622860072954No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13390.14185839798537767No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG12190.1291451733713035No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11920.1262846978331368No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11830.12533120598708125No Hit
AGCCAGGACAGGCCCCTGTGCTTGTCATCTATGGTAACAACAACCGGCCC11690.12384799644877258No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10910.11558440044962437No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10910.11558440044962437No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10870.11516062629582191No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10760.1139952473728651No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10670.11304175552680955No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC10650.1128298684499083No Hit
GTGTATTACTGTGCGAGAAGTAAATGGGACTATGGCTACGGTATGGACGT10600.11230015075765522No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC10370.10986344937329101No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10210.10816835275808111No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT9960.10551976429681564No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9920.10509599014301318No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9820.10403655475850701No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19200.061.290791
ATCAACG19450.058.8525473
TATCAAC19650.057.9424132
TCAACGC19800.057.812224
CAACGCA20300.056.7429245
GGTATCA8200.056.347541
AACGCAG21200.054.3340236
ACGCAGA23500.049.0162247
CGCAGAG24300.047.106268
AGACGGT8350.041.384969
CCCTAAG9850.036.647351
GCAGAGT33500.034.8143239
GTCATAG10200.034.682051
CCTAAGG10000.034.5894242
CTAAGGA10350.034.0834473
TAGGTGG10350.033.3878675
ATAGGTG10950.031.5583934
GTACATG22800.030.7146571
TCTACAC7900.030.0728533
TACATGG22800.029.0773642
Produced by FastQC (version 0.11.5)