FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450442
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGACCCTCAGGTTGTTCAGTTGCAGAGACA27250.18787376537634734No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21330.14705862075146747No Hit
CTCCTGTACAGCGTCTGGATTCACCTTCAGTAACTTTGTCATGCACTGGG14750.10169313905692196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16300.053.8820884
ATCAACG16700.051.7293433
CAACGCA18050.049.05695
GTATCAA18950.049.04021
AACGCAG20100.044.4117436
TATCAAC19750.043.7407652
ACGCAGA21450.041.6165967
GTAATAG12000.040.822481
CGCAGAG22300.038.7390178
CAGCCGT10950.038.131699
ACAGCCG11250.037.7547578
AATAGAC12850.036.9754073
TAGACAG14750.034.1647455
GGTATCA9100.031.6657921
GTACATG33050.031.3879361
GCAGAGT28450.030.1118139
TAATAGA15700.029.804782
ACCGACC5650.029.3056648
ATAGACA17050.029.1337854
TGCACCG6550.028.5761855
Produced by FastQC (version 0.11.5)