FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892598
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA37790.42337087916396854No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31440.35223023130233316No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30570.3424834023827076No Hit
GTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTACTGGGGCCAGGG24670.2763842177553613No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23830.2669734863846883No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23610.2645087710257025No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT23110.2589071452098257No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG21350.23918942233793933No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGCG19690.2205920246292284No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTG18820.21084519570960278No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG17760.1989697489799439No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17580.1969531636862283No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC17490.19594487103937047No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT15130.16950519718843196No Hit
GCTGTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTACTGGGGCCA15000.168048774476304No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA14790.16569609163363574No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG13660.15303641728975417No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13460.15079576696340344No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12990.14553023869647927No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12980.1454182061801617No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12900.14452194604962146No Hit
GATTAAGACTGATGGGAGTGTAACAACCTACGCGGACTCCGTGAAGGGCC12840.14384975095171623No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12210.13679170242371147No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT11090.12424406059614741No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10860.12166731272084409No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGGGACGTT10850.12155528020452656No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTGGATGCACTGGA10810.12110715013925642No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC10800.12099511762293888No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT10670.1195386949108109No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGGCCCCAA10600.11875446729658816No Hit
CTCCTGCACTGGAACCAGCAGGGACGTTGGTGGTTATAACTATGTCTCCT10480.11741007710077773No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10370.11617771942128484No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTGG10320.11561755683969716No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10320.11561755683969716No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT10290.11528145929074454No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGTCCGGGGGAGGCT10150.11371300406229905No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTGT10080.11292877644807628No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC9900.11091219115436064No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9860.1104640610890905No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC9850.11035202857277296No Hit
GTCTGAGAGCCGAGGACACGGCTGTATATTATTGTGCAAGAGATCTTAGG9830.11012796354013787No Hit
GATTATTACTGCAGCTCATCTACAAACAGGAACACTTGGGTGTTCGGCGG9790.10967983347486775No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9710.10878357334432745No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC9550.10699105308324688No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC9490.10631885798534166No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCC9480.10620682546902413No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9460.10598276043638905No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA9410.10542259785480139No Hit
GTCTTAATCTGTGAGACCCACACCGGCCCCTTCCCTGGAGGTTGGCGGAT9350.10475040275689615No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTTGGATCCCC9200.10306991501213313No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT9180.10284584997949804No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9170.10273381746318051No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9020.10105332971841748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18100.060.8553244
ATCAACG18150.060.687673
CAACGCA19100.057.6691745
AACGCAG20000.055.4340256
ACGCAGA21050.053.0109147
CGCAGAG21100.052.54418
GCAGAGT24300.045.9209759
GTGCTAG3150.043.540971
GAATACG2700.042.6620679
TAGGTGG13500.042.128795
ATAGGTG13900.041.9523124
GTGGTCA13650.041.1384248
GTCATAG13750.040.9493451
TATTGAG6350.036.2795565
AGGTGGT16050.034.9868856
CATAGGT17700.033.3523373
TCATAGG17500.032.945812
TGTACCG900.00375505131.9965525
CCGTTGA5400.031.996559
TACATGG19900.031.5075282
Produced by FastQC (version 0.11.5)