FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892598
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30900.34618047542118624No Hit
ATAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA29610.33172828081622413No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24100.2699983643252618No Hit
GTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTACTGGGGCCAGGG22370.250616739002328No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG21120.236612674462636No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20280.227201943091963No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19340.21667088655811462No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16720.1873183672829202No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16440.18418145682602918No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC15340.17185788003110022No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTG15300.17140974996583008No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGCG14840.16625625421522344No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12870.14418584850066885No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12760.14295349082117595No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA12730.1426173932722233No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12680.14205723069063567No Hit
GCTGTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTACTGGGGCCA12450.13948048281533232No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG12090.13544731222790102No Hit
GATTAAGACTGATGGGAGTGTAACAACCTACGCGGACTCCGTGAAGGGCC10750.12043495504135121No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10610.11886649981290569No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG10600.11875446729658816No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT10040.11248064638280614No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9890.11080015863804311No Hit
GATTATTACTGCAGCTCATCTACAAACAGGAACACTTGGGTGTTCGGCGG9680.10844747579537486No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC9480.10620682546902413No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA9460.10598276043638905No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9450.10587072792007152No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTGT9240.10351804507740325No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGGGACGTT9240.10351804507740325No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9150.10250975243054546No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTGGATGCACTGGA9130.10228568739791037No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9050.10138942726737009No Hit
CTCCTGCACTGGAACCAGCAGGGACGTTGGTGGTTATAACTATGTCTCCT9020.10105332971841748No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8990.10071723216946488No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC8950.10026910210419472No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8940.10015706958787719No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)