FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874371
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34910.3992584383516837No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28540.32640606790481386No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGCCAGGACATCAGCAA19740.22576229083535476No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19560.2237036681225704No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19150.2190145830545615No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGTC17980.20563353542146298No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17530.20048697863950202No Hit
ATATGACACTCTTCCTCCCACCTTCGGCGGCGGGACCAAGGTGGACCTCA16050.1835605252232748No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14910.1705225813756403No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14770.168921430376808No Hit
GTAATAAACGGCCGTGTCGTCATTTCTCAGGTTCCTCAGTTCCATGTAAG14120.1614875150250866No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13890.1588570526698621No Hit
CCTTTAAATTGGTATCAACAAAAGCCCGGGAAAGCCCCCAGACTCCTGGT13380.153024288316973No Hit
CTCCTGGTCTATGATGAATTCGATCCGGAAACAGGAATCCCATCAGGATT13100.1498219863193084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.14513290125129952No Hit
CATTTTAGTCTCACACTCAACAGCCTCCACCCTGACGATATTGCAACATA12330.14101565582573072No Hit
GCGTCAGGGTGCTGCTGAGACTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12200.13952887275538645No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11890.13598346697225777No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11840.13541162732981768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA11550.13209495740366503No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11420.13060817433332075No Hit
CAATATGACACTCTTCCTCCCACCTTCGGCGGCGGGACCAAGGTGGACCT11080.126719664764728No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10880.12443230619496758No Hit
GTCCCAACCAGGACACAGCTTGGTCATGAGGGTCCCTGCTCAGCTCCTGG10750.12294552312462331No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10600.12123000419730298No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9620.11002194720547685No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGTCTCAGCAGCACCCTGACGCT9440.10796332449269247No Hit
CTCCCAGGTTGAGCTGGTGCAGTCTGGAGGTGAGGTGAAGAAGCCTGGGG9410.10762022070722839No Hit
GTGCAGCCACAGTTCGTTTGAGGTCCACCTTGGTCCCGCCGCCGAAGGTG9390.10739148485025235No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9350.10693401313630027No Hit
ACATATTTCTGTCAACAATATGACACTCTTCCTCCCACCTTCGGCGGCGG9350.10693401313630027No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9260.10590470177990809No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9030.10327423942468358No Hit
GTCTGGAGGTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTA8990.10281676771073149No Hit
GTGTCATATTGTTGACAGAAATATGTTGCAATATCGTCAGGGTGGAGGCT8950.10235929599677941No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8870.10144435256887521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22100.058.635364
CAACGCA24050.053.881145
AACGCAG25100.051.6271486
ATCAACG27850.046.529323
ACGCAGA28000.046.2800527
CGCAGAG28550.045.3884938
GAATACG3050.042.4866079
GCAGAGT34800.037.4436959
ACCGACC2603.6379788E-1233.2267049
GTGCTAG4400.032.8095741
GTATCAA43050.031.6892471
GTACATG25200.031.5076081
ACATGGG25400.030.893863
TATCAAC45600.028.9126972
CATGGGA13700.028.9015754
ACCGTGT2303.7334758E-828.1704678
GTCTAGA1804.8630227E-628.0704151
GTATAGG1804.8630227E-628.0704151
GTGTAGA5200.027.761951
TCTACAC7300.027.613063
Produced by FastQC (version 0.11.5)