FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874371
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29490.3372710211111759No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22370.2558410560277045No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16730.19133754436046027No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGTC15930.18218811008141853No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15830.1810444307965383No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGCCAGGACATCAGCAA15730.1799007515116581No Hit
ATATGACACTCTTCCTCCCACCTTCGGCGGCGGGACCAAGGTGGACCTCA13110.14993635424779642No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12920.147763363606524No Hit
CTCCTGGTCTATGATGAATTCGATCCGGAAACAGGAATCCCATCAGGATT12840.14684842017861985No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12470.14261680682456304No Hit
GTAATAAACGGCCGTGTCGTCATTTCTCAGGTTCCTCAGTTCCATGTAAG12070.13804208968504217No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11900.1360978349007458No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11720.1340392121879614No Hit
CCTTTAAATTGGTATCAACAAAAGCCCGGGAAAGCCCCCAGACTCCTGGT10960.12534724962287175No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10700.1223736834821832No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10250.11722712670022221No Hit
GCGTCAGGGTGCTGCTGAGACTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10130.11585471155836596No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10060.1150541360589498No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9840.11253804163221333No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9830.1124236737037253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9810.11219493784674928No Hit
CAATATGACACTCTTCCTCCCACCTTCGGCGGCGGGACCAAGGTGGACCT9680.11070815477640499No Hit
CATTTTAGTCTCACACTCAACAGCCTCCACCCTGACGATATTGCAACATA9630.11013631513396487No Hit
GTCCCAACCAGGACACAGCTTGGTCATGAGGGTCCCTGCTCAGCTCCTGG9140.10453228663805181No Hit
GTGCAGCCACAGTTCGTTTGAGGTCCACCTTGGTCCCGCCGCCGAAGGTG9010.10304550356770752No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13350.052.8419274
CAACGCA15200.046.8840835
AACGCAG16050.045.298126
CGCAGAG16950.042.892918
GAATACG3750.042.2304049
ACGCAGA17300.042.0251357
GTGCTAG4300.041.8940771
ATCAACG19700.036.1745223
GCAGAGT20850.035.905519
GTACATG24400.032.485081
GCTAGAG5350.032.291733
TAAACGG8650.031.6228735
CTAGAGA6100.030.6815534
ACATGGG25150.029.480383
TCTACAC9850.027.770343
AACGGCC9400.027.5682057
ATAAACG9800.027.1774984
AAACGGC9550.027.1351956
TGTAGAG7450.027.0542562
GTATAGA2705.9353624E-926.688081
Produced by FastQC (version 0.11.5)