FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821930
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34710.4222987359020841No Hit
ATAATACTCCGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA34160.4156071684936673No Hit
TTATAATACTCCGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGAC29810.36268295353618923No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28400.345528207998248No Hit
ATATTATAATACTCCGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA22980.27958585280985No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG20070.24418137797622672No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18820.22897327022982492No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17880.21753677320453083No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGC17780.21632012458481864No Hit
TTATTACTGTCAGCAATATTATAATACTCCGGTCACTTTCGGCGGAGGGA17190.20914189772851702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17070.20768191938486244No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16670.2028153249060139No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15910.19356879539620164No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15640.19028384412297883No Hit
GTAATACACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTCCAGATATG15360.18687722798778483No Hit
GACCAGGACAGCCTCCTCAGCTGCTCATTTACTGGGCATCTACTCGGGTA14830.1804289903033105No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGGTCACTTTCGGCGGAGG14000.17033080675969975No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13830.1682625041061891No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13830.1682625041061891No Hit
ACTTAGCTTGGTACCGGCAGAGACCAGGACAGCCTCCTCAGCTGCTCATT13780.167654179796333No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13380.16278758531748447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13280.1615709366977723No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT12500.15208107746401764No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12340.1501344396724782No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12110.14733614784714028No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11510.14003625612886741No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11240.13675130485564466No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11040.13431800761622034No Hit
GTCCCTGACCGAGTCACTGGCAGCGGGTCTGGGACTGATTTCACTCTCAC10710.1303030671711703No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10430.1268964510359763No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG10400.12653145645006267No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA10250.12470648352049445No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10070.1225165160050126No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10030.12202985655712774No Hit
ATTATAATACTCCGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGA9820.11947489445573224No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9820.11947489445573224No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9810.11935322959376103No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9780.11898823500784737No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9580.1165549377684231No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9540.11606827832053825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.10986337036000633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8920.10852505687832298No Hit
CTCCTCAGCTGCTCATTTACTGGGCATCTACTCGGGTATCCGGGGTCCCT8910.10840339201635175No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG8650.10524010560510018No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC8560.10414512184735926No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8530.10378012726144563No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT8510.1035367975375032No Hit
GTCAGGGACCCCGGATACCCGAGTAGATGCCCAGTAAATGAGCAGCTGAG8430.10256347864173347No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG8370.10183348946990618No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC8350.10159015974596376No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCA8300.1009818354361077No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8220.10000851654033797No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)