FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800089
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGTCTGTTAGGCTATTCATTTGCAGAGACA41100.5136928516702517No Hit
GTGTATTACTGTGCGAGATCGCAAGCAGGGCTTGACATCTGGCGCGACCC26470.3308381942508896No Hit
CTCCAGAGACAGTGCCAGGAACTCAGTGTCTCTGCAAATGAATAGCCTAA21050.26309573059997077No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19460.24322294144776394No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT18140.22672477686857337No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCTGCATAGTGAATGA17170.21460112562477424No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT17020.21272633419532078No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16820.2102266122893828No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16550.20685198771636654No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16490.20610207114458517No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC14350.17935504675104894No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14220.17773022751218925No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG13430.16785632598373432No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT13310.16635649284017154No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13170.16460668750601495No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12380.15473278597756No Hit
ATACACAGCCGTGTCCTCGTCTGTTAGGCTATTCATTTGCAGAGACACTG12120.15148314749984065No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAGTGCCAGG11550.14435894006791744No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT11460.14323406521024537No Hit
CAGTAATACACAGCCGTGTCCTCGTCTGTTAGGCTATTCATTTGCAGAGA11450.14310907911494847No Hit
ATCATTCACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA11060.13823462139836942No Hit
GTATTACTGTGCGAGATCGCAAGCAGGGCTTGACATCTGGCGCGACCCCT11030.13785966311247874No Hit
CTCCAGACCCCTCCCTGGAGCCTGGCGGACCCAGTGTACGCCAAAAGTAG10880.13598487168302525No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTACCTC10720.1339850941582749No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10170.12711085891694548No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCA10060.1257360118686796No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10040.1254860396780858No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAGTGCCA9970.12461113701100753No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG9870.12336127605803854No Hit
CTCCTGTGCAGCCTCTGGATTCAGCCTCACTACTTTTGGCGTACACTGGG9690.12111152634269437No Hit
GGTCTGGAGTGGGTCTCATACATCGATTATCGTAGTGCTATCATTCACTA9540.1192367349132409No Hit
GTACATGGGAGCTCTCAGAGAGGTGCCTTTGCCCTGGATTCCAAGGCACT9200.11498720767314635No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT9060.1132374023389898No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA9000.1124874857672084No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG8970.1121125274813177No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT8930.11161258310013011No Hit
GCTGTGTATTACTGTGCGAGATCGCAAGCAGGGCTTGACATCTGGCGCGA8800.10998776386127043No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8710.10886288900359833No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT8650.10811297243181696No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA8600.10748804195533246No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCACTACTTTTGGC8600.10748804195533246No Hit
GAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTCCTTGT8580.10723806976473867No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8480.10598820881176971No Hit
GTCTGCATAGTGAATGATAGCACTACGATAATCGATGTATGAGACCCACT8480.10598820881176971No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG8360.10448837566820691No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8350.10436338957291003No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8320.10398843128701932No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8250.10311352861994104No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC8160.10198865376226894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.10173868157167515No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA8010.10011386233281547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG3250.050.9509939
GTGCTAG4200.042.943311
CTAGAGA4250.042.350574
AGAATAC5000.035.9979828
TATACTA2650.035.318785
GTAATAC18750.035.0142561
AATACAC19200.034.8730473
CAGCCGT18950.034.1933369
GCTAGAG5100.033.8804553
TAATACA20550.032.2519262
TGCTAGA5850.032.018222
TATACCG900.003753869631.9982075
ATACCGT1000.006284305428.7983866
GTGTAGA3052.3646862E-1128.3848231
GTCCTAG1804.881418E-628.0562931
GTTAGGA6950.028.027461
GTATGTA7550.027.7112331
TACACAG29700.027.3924065
ACAGCCG35450.026.4019078
GTACACG6600.026.1803515
Produced by FastQC (version 0.11.5)