FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800089
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGTCTGTTAGGCTATTCATTTGCAGAGACA34770.4345766533473151No Hit
GTGTATTACTGTGCGAGATCGCAAGCAGGGCTTGACATCTGGCGCGACCC24440.30546601690561925No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18070.22584987420149505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCAGAGACAGTGCCAGGAACTCAGTGTCTCTGCAAATGAATAGCCTAA18030.2253499298203075No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCTGCATAGTGAATGA15900.19872789152206816No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14840.18547936542059695No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14840.18547936542059695Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14200.17748025532159545No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14030.1753554917015482No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT13770.17210585322382885No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC13050.16310685436245217No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11770.14710863416444922No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11410.14260913473376088No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10960.13698476044540045No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10630.13286021930060282No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10470.13086044177585243No Hit
GTATTACTGTGCGAGATCGCAAGCAGGGCTTGACATCTGGCGCGACCCCT10000.12498609529689822No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT9910.12386122043922614No Hit
ATCATTCACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA9800.12248637339096026No Hit
CAGTAATACACAGCCGTGTCCTCGTCTGTTAGGCTATTCATTTGCAGAGA9670.12086155415210059No Hit
CTCCAGACCCCTCCCTGGAGCCTGGCGGACCCAGTGTACGCCAAAAGTAG9240.11548715205433396No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9230.11536216595903706No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9170.11461224938725566Clontech Universal Primer Mix Long (96% over 26bp)
ATACACAGCCGTGTCCTCGTCTGTTAGGCTATTCATTTGCAGAGACACTG9020.11273745795780218No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCA8950.11186255529072392No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8880.11098765262364561No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAGTGCCA8860.11073768043305182No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT8500.10623818100236349No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAGTGCCAGG8490.1061131949070666No Hit
GTACATGGGAGCTCTCAGAGAGGTGCCTTTGCCCTGGATTCCAAGGCACT8380.10473834785880072No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTACCTC8270.10336350081053482No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT8150.10186366766697205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)