FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807428
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27760.34380774508686845No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA26150.32386788667224814No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAA25020.3098728307663346No Hit
CCCCTGTGCTGATCATCTTTCGAGATACCAAGCGGCCCTCAGGGATCTCT22990.284731270156596No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19450.24088835165488443No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19180.23754440024373688No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18820.23308579836220691No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17600.21797609198591084No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17190.21289823984305722No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16650.20621033702076225No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16270.2015040350347028No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16030.19853163378034944No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA15980.19791238351902582No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15810.19580693263052557No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15310.18961443001728945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15130.18738512907652446No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14720.18230727693367088No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14260.17661017452949365No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTTTTTTGGTATTCGGCGG13890.17202772259569893No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13890.17202772259569893No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13800.17091307212531642No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT13390.16583521998246284No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT12970.1606335177873445No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACTTTTTTGGTATTCGGCGGAG12880.15951886731696202No Hit
GTGCTGATCATCTTTCGAGATACCAAGCGGCCCTCAGGGATCTCTGAGCG12770.15815651674205006No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12310.15245941433787286No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG12300.15233556428560813No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12290.1522117142333434No Hit
TCCATAGTCTGGGTCCCGCTGATGGTCAGAGTGGCTGTATTCCCAGAGTT11900.14738156219501924No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11710.14502841120198953No Hit
GACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTT11490.14230371005216563No Hit
GTAATAGTCAGCCTCATCCATAGTCTGGGTCCCGCTGATGGTCAGAGTGG11180.13846435843195926No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11170.13834050837969453No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10750.13313880618457619No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG10650.131900305661929No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC10200.1263270533100165No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAAAAGTGCTG10150.1257078030486929No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10120.12533625289189873No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10070.12471700263057511No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAAAAGTGCTGC9680.11988685059225095No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9440.11691444933789762No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG9390.116295199076574No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9280.11493284850166206No Hit
CAGTAATAGTCAGCCTCATCCATAGTCTGGGTCCCGCTGATGGTCAGAGT9260.11468514839713263No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA9250.11456129834486792No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8840.10948344620201429No Hit
GCAGAAGGCAGGCCAGTCCCCTGTGCTGATCATCTTTCGAGATACCAAGC8660.10725414526124931No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTC8590.10638719489539623No Hit
TCTTTCGAGATACCAAGCGGCCCTCAGGGATCTCTGAGCGATTCTCTGGC8550.10589179468633736No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG8540.10576794463407263No Hit
GCCCTCAGGGATCTCTGAGCGATTCTCTGGCTCCAACTCTGGGAATACAG8400.10403404390236654No Hit
CTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTTTT8210.10168089290933681No Hit
GTCTGGGTCCCGCTGATGGTCAGAGTGGCTGTATTCCCAGAGTTGGAGCC8100.10031854233442487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22900.062.676071
ATCAACG24750.057.390563
TCAACGC24850.057.159614
TTTCGCG1206.366463E-1153.9863439
CAACGCA26700.053.1991125
AACGCAG27300.051.9557766
TATCAAC27400.051.840012
CGCAGAG29650.047.826018
ACGCAGA30050.047.1893927
GCAGAGT34900.041.4565629
CATTTCG1854.483809E-935.018177
GTACATG26200.031.9330841
ATTTCGC2051.22036E-831.6017638
TATTGAG15700.030.7697935
AGGCCGT2851.0913936E-1130.3156326
CAGAGTA34550.029.5844149
TACATGG27500.029.3653222
ACATGGG27950.028.8925323
GAATACG2502.6411726E-928.7927199
GTGCTAG3850.028.100541
Produced by FastQC (version 0.11.5)