FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807428
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29220.3618898527175179No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAA24290.3008317769510099No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA22160.2744517158186241No Hit
CCCCTGTGCTGATCATCTTTCGAGATACCAAGCGGCCCTCAGGGATCTCT21170.2621905606444166No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18500.22912259668973584No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18120.22441629470367638No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17740.21970999271761693No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16120.19964628425073197No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15800.19568308257826086No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15620.19345378163749585No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14730.18243112698593558No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14680.181811876724612No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14470.17921102562705282No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14450.17896332552252336No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14280.1768578746340231No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13560.16794067087096312No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13450.16657832029605116No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTTTTTTGGTATTCGGCGG12890.15964271736922672No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12730.15766111653299117No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACTTTTTTGGTATTCGGCGGAG12200.1510970637629609No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12050.14923931297899007No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11910.14750541224728397No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11860.14688616198596036No Hit
GACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTT11790.1460192116201073No Hit
GTGCTGATCATCTTTCGAGATACCAAGCGGCCCTCAGGGATCTCTGAGCG11720.14515226125425426No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT11590.14354221057481287No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11530.14279911026122452No Hit
TCCATAGTCTGGGTCCCGCTGATGGTCAGAGTGGCTGTATTCCCAGAGTT11340.1404459592681948No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11280.1397028589546065No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11050.13685430775251786No Hit
GTAATAGTCAGCCTCATCCATAGTCTGGGTCCCGCTGATGGTCAGAGTGG10820.13400575655042926No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10800.1337580564458998No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG10220.12657475341454594No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9950.12323080200339845No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAAAAGTGCTGC9390.116295199076574No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAAAAGTGCTG9360.11592364891977985No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9320.11542824871072095No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC9230.11431359824033846No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG8980.1112173469337204No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG8730.10812109562710236No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8420.10428174400689597No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTC8340.10329094358877819No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8340.10329094358877819No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8290.10267169332745457No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA8160.1010616426480132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG15050.059.2450033
TCAACGC15100.059.0488364
GTATCAA14950.058.690271
TATCAAC17100.051.304712
CAACGCA18300.049.1101845
ACGCAGA18700.046.96757
CGCAGAG19100.045.983898
AACGCAG19850.045.2501036
GCAGAGT21500.041.1856469
GTACATG26350.035.2092321
TCCGCTA2302.910383E-1134.430568
TATTGAG15400.034.0811965
TACATGG26900.033.6830832
ACATGGG27450.033.2680973
TACACCG950.00492372530.272555
TTATTGA18900.028.5342244
ATCCGCT3052.5465852E-1128.3243967
ATTGAGT11700.027.637376
GTGCTAG4450.027.4749181
GAATACG3700.027.2399029
Produced by FastQC (version 0.11.5)