FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848942
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATGTAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGAAAAA34340.40450348787078505No Hit
ACATATTACTGTGCGAGCGACTGGCACGGCGGCTATTGGGGCCAGGGAAC24530.2889478904330331No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20540.24194821318770893No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17730.2088481898645608No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15970.1881165026586033No Hit
TTTAAGAGGTGTCCAGTGTCAGGAGCAGTTGGTGGAGTCTGGGGGAGGCG13720.16161292526462348No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13600.16019940113694459No Hit
GATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAG13300.15666559081774725No Hit
TACTTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT12890.1518360500481776No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12790.15065811327511183No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12530.14759547766514083No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11510.13558052257987002No Hit
CTGTAGGACAGCCGGGAAGATGTGCACGCCGCTGGTCAGGGCGCCTGAGT11000.1295730450372346No Hit
ATACTACACGGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT10990.129455251359928No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10930.12874848929608854No Hit
CCATAGTAGAGCAGGCAGTAATAATCACCCTCGTCCTCAGGCTGCACATG10910.1285129019414754No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10700.12603923471803727No Hit
GCATAAGTATTGAAGGTGAATCCAGAGGCTTCACAAGAGAGTCTCAGGGA10340.1217986623350005No Hit
ACTAAATACTACACGGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA10320.12156307498038736No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9860.11614456582428481No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9410.11084385034548884No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG9080.10695665899437182No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC8870.1044829917709337No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8830.1040118170617074No Hit
CTACTATGGTGGTGCCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCC8820.1038940233844008No Hit
CAGTAATATGTAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGAAA8720.10271608661133506No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8650.101891530870189No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8600.10130256248365613No Hit
GTGTAGTATTTAGTAGTTTCATCATATGAAATAACTGCCACCCACTCCAG8500.10012462571059036No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8490.10000683203328377No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8490.10000683203328377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15350.043.6533781
ATCAACG15300.042.3732643
TCAACGC15500.041.8215874
TATCAAC16650.039.3748782
TAGCCGT13850.037.4277049
CAACGCA17800.036.4176675
GTAGCCG14350.036.1257328
AACGCAG18200.035.602596
GTACACG9150.035.4226535
CGCAGAG18350.035.313648
ACACGGG9550.034.6788677
ATGTAGC16250.032.3429766
GTACATG26450.032.1439821
TGTAGCC16400.031.608157
TACATGG26700.031.0297782
GTAATAT17000.030.9397811
TATGTAG17550.030.7803655
TAATATG17200.030.1575552
ACGGGGT10750.030.1380049
CTGTGCG13950.029.9339649
Produced by FastQC (version 0.11.5)