FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852705
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15960.1871690678487871No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15740.1845890431040043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15690.1840026738438264No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14280.1674670607068095No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12680.14870324438111657Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12220.14330864718747985No Hit
GTGTATTACTGTGCGAGAGTGGGGGAGTATAGCAGCAGCTGGTATTCCCC10170.11926750752018575No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10100.11844659055593669No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG10060.11797749514779438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)