FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852705
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17190.20159375164916352No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15940.18693452014471595No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14640.17168891938009043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12540.14706141045261842Clontech Universal Primer Mix Long (96% over 26bp)
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12520.14682686274854725No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11390.13357491746852662No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10020.11750839973965206No Hit
GTGTATTACTGTGCGAGAGTGGGGGAGTATAGCAGCAGCTGGTATTCCCC9480.11117561172973069No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8840.1036700851994535No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT8720.10226279897502653No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)