FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962520
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAGACTCTCCTGTAGAGCCTCTGGATTCACTTTCGATCAGGCCTGG28810.2993184557204006No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27330.2839421518513901No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27010.2806175456094419No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGGCT24640.25599468063001285No Hit
CTCCTGTAGAGCCTCTGGATTCACTTTCGATCAGGCCTGGATGACCTGGG24560.25516352906952583No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC23950.24882599842081204No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23780.24705980135477706No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22180.23043677014503594No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21850.22700826995802684No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21600.2244109213315048No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21360.22191746665004364No Hit
GTACAATAATAAACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATCTGCAG20760.2156838299463907No Hit
GGCCTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG19860.20633337489091136No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19650.20415160204463284No Hit
TTATTGTACCACACCACCTCGTGTAGTGGGAAGTAGAGTCGGGGACTATT19580.20342434442920665No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGA18390.19106096496696173No Hit
GTCTGTAGTCGCACCTTCAGTTTTGCCTTTAATACGGCCAACCCACTCCA18100.18804804056019614No Hit
CCTGTAGAGCCTCTGGATTCACTTTCGATCAGGCCTGGATGACCTGGGTC18050.18752857083489174No Hit
GTACCACACCACCTCGTGTAGTGGGAAGTAGAGTCGGGGACTATTGGGGC16710.1736067821967336No Hit
ATAATAAACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATCTGCAGATACA16250.16882766072393302No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG16220.16851597888875036No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT15620.16228234218509746No Hit
TATATTAGCAGCACCACTCTCGAGGTTTTCGGCGGAGGGACCAAGGTGAC15470.16072393300918422No Hit
GTCTAAGGGACCCGCCCGGCTTTACCAAGCCTCCCCCAGACTCAACCAGC15410.16010056933881892No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15080.15667206915180984No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14740.15313967501973985No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14700.15272409923949634No Hit
GTGTGGTACAATAATAAACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATC14540.1510617961185222No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14510.15075011428333956No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA14230.14784108382163488No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG14160.1471138262062087No Hit
ATCCAGGCCTGATCGAAAGTGAATCCAGAGGCTCTACAGGAGAGTCTAAG14120.14669825042596518No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13900.14441258363462578No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13820.1435814320741387No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13650.14181523500810372No Hit
ACTACAGACTACGCTGCACCCGTGGATGACAGATTCATCATCTCAAGAGA13210.13724390142542492No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13130.13641274986493787No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT12980.13485434068902463No Hit
GTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGGCTTGGTAAAGCCGGGCG12950.134542658853842No Hit
GCAGATACAGCGTGTTTTTCGCATCATCTCTTGAGATGATGAATCTGTCA12800.13298424967792877No Hit
GCTAATATATGCGCGGCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGGC12540.13028300710634583No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12380.12862070398537173No Hit
GACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCTCGAGAGTGGTG12370.12851681004031085No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC12190.12664671902921498No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12170.1264389311390932No Hit
CTACAGGAGAGTCTAAGGGACCCGCCCGGCTTTACCAAGCCTCCCCCAGA12110.12581556746872793No Hit
ACACGAGGTGGTGTGGTACAATAATAAACGGCTGTGTCCTCGGTTTTCAG11960.1242571582928147No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGATTTTC11850.123114324897145No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA11730.12186759755641441No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11640.12093255205086649No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11610.12062087021568384No Hit
GAGTTTGGACTGAGCTGGATTTTCCTTGCTGCTCTTTTAAAAGGTGTCCA11270.11708847608361385No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGT11180.11615343057806592No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11050.11480280929227445No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11030.11459502140215268No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG10590.11002368781947389No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10360.1076341270830736No Hit
GTTTATTATTGTACCACACCACCTCGTGTAGTGGGAAGTAGAGTCGGGGA10190.1058679300170386No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC10040.10430952084112537No Hit
AGGTGGTGTGGTACAATAATAAACGGCTGTGTCCTCGGTTTTCAGGCTGT9950.10337447533557745No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9790.10171217221460332No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA9780.10160827826954245No Hit
CTATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGG9740.10119270248929893No Hit
GTATCTGCAGATGAACAGCCTGAAAACCGAGGACACAGCCGTTTATTATT9730.10108880854423803No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC9690.10067323276399451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5350.080.7781451
GTATCAA14900.077.8280261
TCAACGC16150.069.994774
ATCAACG16450.068.721843
CAACGCA17050.066.2965855
TATCAAC17400.065.38362
AACGCAG18150.062.6752856
CGCAGAG20000.057.2348338
GCAGAGT23100.049.865639
TAGACGG750.001534564738.4005244
GTACACG11300.038.2286265
TACACGG11450.037.7278146
GTATGTA12800.037.701731
ACGCAGA30750.037.2259147
ACACGGG12250.035.2621237
ACGGGGT12500.034.5568859
TATGTAC13500.033.068842
TAGCACC2258.5310603E-1032.000444
TACATGG20850.031.771872
CATGGGA9750.031.754284
Produced by FastQC (version 0.11.5)