FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962520
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34590.3593691559655903No Hit
CCCTTAGACTCTCCTGTAGAGCCTCTGGATTCACTTTCGATCAGGCCTGG28910.3003573951710094No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26270.2729293936749366No Hit
CTCCTGTAGAGCCTCTGGATTCACTTTCGATCAGGCCTGGATGACCTGGG25850.2685658479823796No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC24580.2553713169596476No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGGCT24270.25215060466276024No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23790.24716369529983792No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22220.23085234592527948No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21760.2260732244524789No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20200.20986576902298132No Hit
GGCCTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG19680.2044632838798155No Hit
CCTGTAGAGCCTCTGGATTCACTTTCGATCAGGCCTGGATGACCTGGGTC19370.20124257158292816No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19240.1998919502971367No Hit
GTACAATAATAAACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATCTGCAG19190.1993724805718323No Hit
TTATTGTACCACACCACCTCGTGTAGTGGGAAGTAGAGTCGGGGACTATT19090.19833354112122345No Hit
ACTACAGACTACGCTGCACCCGTGGATGACAGATTCATCATCTCAAGAGA18670.19396999542866641No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18270.18981423762623115No Hit
GTCTGTAGTCGCACCTTCAGTTTTGCCTTTAATACGGCCAACCCACTCCA17770.18461954037318706No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGA17590.18274944936209117No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17350.18025599468063No Hit
GTCTAAGGGACCCGCCCGGCTTTACCAAGCCTCCCCCAGACTCAACCAGC17190.1785936915596559No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16550.17194447907575947No Hit
TATATTAGCAGCACCACTCTCGAGGTTTTCGGCGGAGGGACCAAGGTGAC15950.16571084237210656No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15850.16467190292149772No Hit
GTACCACACCACCTCGTGTAGTGGGAAGTAGAGTCGGGGACTATTGGGGC15290.15885384199808833No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15010.15594481153638365No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15010.15594481153638365No Hit
ATCCAGGCCTGATCGAAAGTGAATCCAGAGGCTCTACAGGAGAGTCTAAG14910.15490587208577483No Hit
ATAATAAACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATCTGCAGATACA14470.15033453850309603No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14400.14960728088766986No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14240.14794497776669577No Hit
CTACAGGAGAGTCTAAGGGACCCGCCCGGCTTTACCAAGCCTCCCCCAGA14230.14784108382163488No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13990.1453476291401737No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT13850.14389311390932136No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG13590.14119187133773844No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC13510.14036071977725137No Hit
GTGTGGTACAATAATAAACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATC13180.1369322195902423No Hit
GTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGGCTTGGTAAAGCCGGGCG12790.13288035573286788No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12690.13184141628225907No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12360.12841291609524996No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12270.12747787058970203No Hit
GCTAATATATGCGCGGCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGGC12000.12467273407305822No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11930.12394547645763206No Hit
GACTTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCTCGAGAGTGGTG11360.1180235215891618No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11200.11636121846818769No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGATTTTC11190.11625732452312679No Hit
GAGTTTGGACTGAGCTGGATTTTCCTTGCTGCTCTTTTAAAAGGTGTCCA10880.11303661222623945No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10880.11303661222623945No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10860.1128288243361177No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10740.11158209699538711No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10740.11158209699538711No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG10730.11147820305032621No Hit
GTTTATTATTGTACCACACCACCTCGTGTAGTGGGAAGTAGAGTCGGGGA10640.1105431575447783No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT10480.10888085442380417No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10450.10856917258862155No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGT10320.10721855130283006No Hit
ACACGAGGTGGTGTGGTACAATAATAAACGGCTGTGTCCTCGGTTTTCAG10260.10659518763246477No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10140.10534846029173421No Hit
GCAGATACAGCGTGTTTTTCGCATCATCTCTTGAGATGATGAATCTGTCA10010.10399783900594273No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9930.10316668744545568No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC9800.10181606615966422No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG9740.10119270248929893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.064.536411
TCAACGC8100.060.465184
ATCAACG8700.057.128983
CAACGCA9600.051.014845
TATCAAC10500.050.0821272
AACGCAG10150.048.2329336
CGCAGAG10500.046.6251758
GGTATCA3600.038.026871
GCAGAGT13700.035.732769
TACATGG17050.034.2223472
GTACATG18150.033.742851
ACATGGG17750.031.6535823
TTATTGA9450.029.7244784
TATTGAG9450.028.1986795
GTATAGG2853.4742698E-1027.8091281
CTGTGCG1050.007977927.4251489
GTATGTA13700.027.3477941
TACACGG12100.026.7749086
GGCAGCG10050.026.505537
GTACACG12300.026.3491295
Produced by FastQC (version 0.11.5)