FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741106
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA38310.5169301017668188No Hit
GTGTATTACTGTGCGAGAGACTTTTATGGTTCAGGGAGTTATGGGCTCAT26330.35527981152493704No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGA26220.3537955434175408No Hit
GTATATGTAACTACTACTACTACTAATGGATGAGACCCACTCCAGCCCCT20900.2820109404052861No Hit
GTATAATAACTGGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA19300.2604215861158862No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18030.2432850361486751No Hit
ATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG14610.1971377913550828No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13600.18350951145989913No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13240.17865190674478415No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13170.1777073724946229No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13040.17595323745860916No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11330.15287961506181302No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10930.14748227648946305No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT10900.1470774760965368No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10820.1459980083820668No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG10480.14141027059556932No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC9940.13412386352289687No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9930.1339889300585881No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9820.13250466195119187No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9780.13196492809395688No Hit
GCGTAGTATATGTAACTACTACTACTACTAATGGATGAGACCCACTCCAG9770.13182999462964812No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG9470.12778199070038565No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA9260.1249483879499019No Hit
GTATTACTGTGCGAGAGACTTTTATGGTTCAGGGAGTTATGGGCTCATTG9230.12454358755697564No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA8750.11806678127015569No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8700.11739211394861193No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8610.11617771276983319No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8590.1159078458412157No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGCATGAACTGGG8320.11226464230487947No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG8240.11118517459040947No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCAAGCCTGGGG8100.10929610609008698No Hit
TTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCC7830.10565290255375075No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA7810.10538303562513324No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7600.10254943287464952No Hit
GCTGTGTATTACTGTGCGAGAGACTTTTATGGTTCAGGGAGTTATGGGCT7530.10160489862448827No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7430.10025556398140079No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)