FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925344
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG20380.22024241795483623No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG19140.20684199605768236No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTACA16080.17377321298889928No Hit
GTGTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA15610.16869402081820384No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14420.15583393851367708No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGCTGCTG10280.11109382024414705No Hit
CCATGTACACTGTGCTCGTGGACGTGTCCCTTCTCATGGTGACTCTGCCC9350.1010435038212816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.065.942861
TCAACGC13550.058.9103664
ATCAACG14050.056.8016133
AACGCAG15500.051.974276
TATCAAC15700.050.8320162
CGCAGAG17100.047.5318158
ACGCAGA18250.044.1425327
GGTATCA6100.034.212271
GTACACG5700.034.0659455
ACATGGG26550.031.4128593
CAACGCA26800.029.7865165
ACACGGG7000.027.7439177
GTCTAAC1607.18148E-526.98641
GTACTAG1959.272715E-625.8331361
GCAGAGT34200.024.8174959
ATACACG10750.024.7514884
GTATGTA11700.024.6029871
CATGGGG17250.024.5963654
CATGGGA13450.024.5948164
TATGTAC8200.024.550382
Produced by FastQC (version 0.11.5)