FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278261
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAAATCTCAGACTTCTCAGTTCCATGTAGA7210.2591092535425374No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT6830.2454530099439016No Hit
GTCTACATGGAACTGAGAAGTCTGAGATTTGAGGACACGGCCGTATATTA6460.23215614117680883No Hit
GTATATTACTGTGCGGGATCATTACAATCCACAATTTCGTCTTCGGGCGG6190.22245302072514653No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6070.21814052274662996No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCA4920.17681241711917947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.17357804363529206No Hit
GTATGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAGCCTCC4740.1703436701514046No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4390.15776555104739795No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4340.15596867688968272No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC4290.15417180273196746No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC4250.15273430340579527No Hit
GTCTACAAGATCACAATTACCCGTGGACGTTCGGCCAAGGGACCAAGGTG4120.14806243059573565No Hit
GTCTGTATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG4100.14734368093264957No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA4080.14662493126956347No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC3970.14267180812258995No Hit
CATGTAGACTGTGCTCGTGGATTCGTCCGCGGTGATCGTGACTCTGCCCT3900.1401561843017886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3890.13979680947024556No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC3830.13764056048098727No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA3650.13117181351321242No Hit
GCCGTATATTACTGTGCGGGATCATTACAATCCACAATTTCGTCTTCGGG3620.1300936890185833No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAA3580.12865618969241108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3480.1250624413769806No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG3440.12362494205080841No Hit
ATATTACTGTGCGGGATCATTACAATCCACAATTTCGTCTTCGGGCGGTA3170.11392182159914613No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG3060.1099686984521726No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG2960.10637495013674213No Hit
ACTTATTACTGTCTACAAGATCACAATTACCCGTGGACGTTCGGCCAAGG2940.10565620047365604No Hit
GATCTATGCTGCATCCAGGTTGCAAAGTGGGGTCCCATCCAGGTTCCGCG2890.10385932631594079No Hit
ATATACGGCCGTGTCCTCAAATCTCAGACTTCTCAGTTCCATGTAGACTG2790.10026557800051031No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2790.10026557800051031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6450.060.361811
TCAACGC7350.052.922934
ATCAACG7550.052.475093
CAACGCA7750.050.1914255
TATCAAC8150.048.61192
AACGCAG8000.048.5879946
CGCAGAG8900.043.6667488
ACGCAGA9500.041.666427
CAGCGCT1452.0478183E-839.7071849
TACGGCC3650.037.4634557
TATACGG3500.037.0460555
GCTATAA800.002090245736.0494161
ATACGGC3600.035.99116
TCTACAT4200.034.3019032
ATGGAAC4200.034.271087
ACGTGCT3900.033.246463
GCTAATA1102.5254578E-432.7427252
CTACATG4450.032.3748253
GTAATAT4050.032.0439221
TAATATA4500.030.4143522
Produced by FastQC (version 0.11.5)