FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278261
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAAATCTCAGACTTCTCAGTTCCATGTAGA7040.2529998814063056No Hit
GTATATTACTGTGCGGGATCATTACAATCCACAATTTCGTCTTCGGGCGG5880.21131240094731207No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT5880.21131240094731207No Hit
GTCTACATGGAACTGAGAAGTCTGAGATTTGAGGACACGGCCGTATATTA5740.2062811533057094No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5100.18328116408695433No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4490.16135929936282845No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCA4350.15632805172122577No Hit
GTATGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAGCCTCC4110.14770305576419263No Hit
GTCTACAAGATCACAATTACCCGTGGACGTTCGGCCAAGGGACCAAGGTG3940.14159368362796082No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT3770.135484311491729No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC3680.13224993800784154No Hit
CATGTAGACTGTGCTCGTGGATTCGTCCGCGGTGATCGTGACTCTGCCCT3640.13081243868166936No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA3630.13045306385012634No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA3580.12865618969241108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3560.127937440029325No Hit
GTCTGTATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG3360.12074994339846402No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC3260.11715619508303354No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC3250.11679682025149052No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC3220.11571869575686138No Hit
ATATTACTGTGCGGGATCATTACAATCCACAATTTCGTCTTCGGGCGGTA2980.1070936997998282No Hit
GCCGTATATTACTGTGCGGGATCATTACAATCCACAATTTCGTCTTCGGG2950.10601557530519906No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2950.10601557530519906No Hit
ATATACGGCCGTGTCCTCAAATCTCAGACTTCTCAGTTCCATGTAGACTG2940.10565620047365604No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT2930.10529682564211298No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG2910.1045780759790269No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAA2810.1009843276635964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA3900.059.0905231
TCAACGC4250.047.3779224
ATCAACG4300.046.8270153
CAACGCA4350.046.2971045
AACGCAG4600.043.7730836
TATCAAC5250.041.0930942
CATTAGC1701.9699655E-938.071558
TACACCG800.002115032635.962935
ATAGAGC1001.4568976E-435.956463
TGATAAC850.002849956333.8413733
TACACTG2404.7293724E-1132.966025
CAGCGCT1751.0790973E-732.8744749
ACATTAG2258.58563E-1031.9612987
CGCAGAG6100.031.8303078
ACACAGT2507.6397555E-1131.6416846
ACGCAGA6300.030.819827
GCCATAC950.00487852230.3227671
ACGAGTG950.004908697730.2845735
GTGCACC950.004913020430.2791234
GCAGCGC1902.2201493E-730.2791238
Produced by FastQC (version 0.11.5)