FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098272
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.050.8100741
ATCAACG20750.047.4749153
TCAACGC20850.047.2515264
ACGCAGA25100.040.1175967
CGCAGAG25400.039.6437648
CAACGCA25350.039.1473585
AACGCAG26550.037.9266136
TATCAAC27750.037.3130872
GTACATG37900.036.085431
TACATGG37800.035.0015872
ACATGGG37550.033.5111853
GCAGAGT32400.031.0787539
GGTATCA12500.029.3682231
AATCCGC3659.094947E-1225.6171676
CATGGGG21850.023.3672834
GTATACG1902.3026986E-422.7308221
TATACTG11000.022.2273435
AAATCCG4308.0035534E-1121.7408335
ATGGGGC7300.021.6721085
CTAGTAC4058.931238E-1021.3053153
Produced by FastQC (version 0.11.5)