FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347025
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAGAGGGGTGCATTGTGATGGTGGTAGTTGCTACCC39020.28967539577958834No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC32910.24431617824465027No Hit
GTAATAGACAGCCGTGTCCCCGACTCTCAGACTGTTCATCTGCAGATACA31880.23666969803826954No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27380.20326274568029548No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25900.19227557023811734No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21760.1615411740687812No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21720.16124422338115477No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21650.1607245596778085No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA19590.14543159926504706No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19270.14305599376403555No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18400.13659731630816058No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17920.13303390805664334No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17340.12872812308606002No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16270.12078469219205284No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15180.11269278595423247No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG15100.11209888457897961No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15020.11150498320372673No Hit
CTCCTGTCCTGGTCATCTATCAGGATAGCCAGCGGCCATCAGGGATCCCT15010.11143074553182013No Hit
GTCTGAGAGTCGGGGACACGGCTGTCTATTACTGTGCGAGAGGGGTGCAT14990.1112822701880069No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14750.1095005660622483No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14130.10489783040403852No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14100.1046751173883187No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13990.103858502997346No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC13720.10185408585586755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13630.10118594680870807No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13550.10059204543345522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25050.069.947931
ATCAACG30400.056.6239553
TCAACGC30950.055.6177144
CAACGCA31800.054.1290635
AACGCAG33650.051.3290636
TATCAAC36550.047.884482
CGCAGAG36600.047.3885238
CAGCGCT8500.043.180589
GCAGCGC8900.042.857138
GCAGAGT43400.040.1294179
ACGCAGA45150.038.2552227
GTACATG41200.036.228421
GGCAGCG10450.035.8116467
CAGCCGT15300.035.2782489
TACATGG41150.035.0054782
ACATGGG41450.034.057083
CAGGGCA12450.032.3962364
GTACACG15500.031.596355
ATAGACA18300.031.4858024
TACACGG15900.030.778616
Produced by FastQC (version 0.11.5)