FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347025
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAGAGGGGTGCATTGTGATGGTGGTAGTTGCTACCC37390.2775746552588111No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC32050.23793173846068189No Hit
GTAATAGACAGCCGTGTCCCCGACTCTCAGACTGTTCATCTGCAGATACA28160.20905328408901097No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27370.20318850800838886No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26980.2002932388040311No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21660.1607987973497151No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20230.15018281026707003No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19270.14305599376403555No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19130.14201666635734303No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17850.1325142443532971No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16940.12575861620979567No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA16900.12546166552216922No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16500.12249215864590486No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16170.12004231547298677No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG16150.11989384012917353No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15830.11751823462816206No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15070.11187617156325977No Hit
CTCCTGTCCTGGTCATCTATCAGGATAGCCAGCGGCCATCAGGGATCCCT14440.1071991982331434No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14190.10534325643547819No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13670.10148289749633453No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13580.10081475844917503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19800.066.170331
ATCAACG21400.059.136453
TCAACGC22250.056.5583464
CAACGCA23750.052.6912965
AACGCAG24750.050.2717676
TATCAAC27250.047.232712
CGCAGAG27650.045.259258
CAGCGCT11650.043.8314329
GGCAGCG11550.042.3428577
GCAGCGC12850.040.857618
GCAGAGT31950.038.9429139
ACGCAGA33700.037.134077
CAGGGCA15350.034.1981934
CAGCCGT14250.033.8152969
GTACATG42750.033.004791
TACATGG41700.032.2449652
ACATGGG41950.030.8529663
GTACACG18650.030.4650235
TACACGG19850.028.9856286
AGCGCTG11050.028.6380635
Produced by FastQC (version 0.11.5)