FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600641
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGGGTAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGACCG36520.6080171017296522No Hit
CCCATATAGAGCACACAGTAATAATCAGATTCATCATGTGCCTGGGCCCC30760.512119552278316No Hit
GATTATTACTGTGTGCTCTATATGGGTAGTGGCATTTCGGTGTTCGGCGG23770.3957438802878925No Hit
GTAATAATCAGATTCATCATGTGCCTGGGCCCCCGTGATGGTGAGGGCTG21960.3656094072832191No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20670.3441323519373469No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18400.3063393940806572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.28652722674609293No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCAC17160.28569478274043897No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTA16720.27836927549068413No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGA16590.2762049210759838No Hit
CCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15270.25422839932671926No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14950.24890075769053396No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14110.23491569839554743No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13900.2314194335718008No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13800.22975454556049285No Hit
CTCATCTACAGCACAAGCACTCGCCGCTCTGGGGTCCCTGATCGCTTCTC13450.22392743752091515No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13160.2190992622881222No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA12780.2127726878451521No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTTA12480.20777802381122834No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA12090.20128496056712747No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11980.19945358375468872No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT11460.19079616609588756No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11440.19046318849362598No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT11140.18546852445970222No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10880.18113981563030163No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT10840.18047386042577845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.17847599481220897No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10530.17531270759072393No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA9730.16199360350026057No Hit
TTATTACTGTGTGCTCTATATGGGTAGTGGCATTTCGGTGTTCGGCGGAG9580.15949627148329867No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC9520.15849733867651392No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG9440.15716542826746757No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9390.15633298426181363No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCC9370.15600000665955205No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTGCTTACTACCCCAGCTGG9260.15416862984711333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.1530032082391978No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGT9060.1508388538244975No Hit
GTACATGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTCATGAGTGTCCC9040.1505058762222359No Hit
CAGTAATAATCAGATTCATCATGTGCCTGGGCCCCCGTGATGGTGAGGGC8910.14834152180753563No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAGCACTCGCCGC8890.148008544205274No Hit
CACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGAGGGCGGGAACA8810.1466766337962277No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT8650.14401281297813504No Hit
CATCTACAGCACAAGCACTCGCCGCTCTGGGGTCCCTGATCGCTTCTCTG8630.14367983537587345No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGA8240.13718677213177255No Hit
GGTATCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACA8210.13668730572838017No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTAC8110.13502241771707227No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8050.1340234849102875No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTTAATTCT8030.13369050730802592No Hit
CTCTACTGCTTACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGGCTC7870.13102668648993326No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7820.1301942424842793No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7800.1298612648820177No Hit
ATATGGGTAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTC7750.12902882087636375No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG7530.12536606725148633No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT7510.12503308964922474No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7430.1237011792401784No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC7370.12270224643339367No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG7330.12203629122887048No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6940.11554322798476961No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6890.11471078397911563No Hit
CTACTGCTTACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGGCTCCA6820.1135453623712001No Hit
ACCCATATAGAGCACACAGTAATAATCAGATTCATCATGTGCCTGGGCCC6700.1115474967576306No Hit
GCTCTATATGGGTAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGA6680.11121451915536902No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGG6610.11004909754745347No Hit
ATATAGAGCACACAGTAATAATCAGATTCATCATGTGCCTGGGCCCCCGT6540.10888367593953792No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6540.10888367593953792No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6540.10888367593953792No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6500.10821772073501476No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA6390.10638634392257605No Hit
GCTTACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGGCTCCACGCAC6150.10239061269543703No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6010.10005976947960596No Hit
ATAGAGCACACAGTAATAATCAGATTCATCATGTGCCTGGGCCCCCGTGA6010.10005976947960596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGC300.002218553870.046356
GTATCAA21800.066.5118941
TATCAAC24800.058.4661032
ATCAACG25450.057.5233273
TCAACGC25750.056.8531534
CAACGCA26450.055.348535
AACGCAG27200.053.8223766
CGCAGAG31000.046.9988378
ACGCAGA32550.044.9759947
GCAGAGT33350.043.267019
GACCGTT658.689272E-443.1054427
GTATTAG1452.6086127E-838.646261
TCTATTA3450.038.576252
GTACATG24750.038.20711
TACATGG24850.038.0533452
CTATTAC3500.038.025163
TTAGGAC1159.039299E-636.545923
ACATGGG25800.036.380663
CATGGGA9900.036.084484
ATGGGAG7000.036.0238345
Produced by FastQC (version 0.11.5)