FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600641
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGGGTAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGACCG37600.6259978922517777No Hit
CCCATATAGAGCACACAGTAATAATCAGATTCATCATGTGCCTGGGCCCC30360.5054600002330844No Hit
GATTATTACTGTGTGCTCTATATGGGTAGTGGCATTTCGGTGTTCGGCGG24280.4042348091455628No Hit
GTAATAATCAGATTCATCATGTGCCTGGGCCCCCGTGATGGTGAGGGCTG22870.38075988818612116No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21200.3529562583972789No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCAC17560.29235433478567063No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17050.28386340592800025No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16970.2825314955189539No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGA15590.25955604096290463No Hit
CCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14810.24656991447470286No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTA14620.24340662725321782No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13490.22459339272543835No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13310.22159659430508408No Hit
CTCATCTACAGCACAAGCACTCGCCGCTCTGGGGTCCCTGATCGCTTCTC12730.2119402438394981No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12480.20777802381122834No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12240.20378229258408934No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTTA12140.2021174045727814No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA12120.20178442697051982No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11950.19895411735129637No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA11610.19329349811284943No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT11310.18829883407892567No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10430.17364781957941597No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT10350.17231590917036965No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT10310.17164995396584648No Hit
TTATTACTGTGTGCTCTATATGGGTAGTGGCATTTCGGTGTTCGGCGGAG10290.1713169763635849No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT10040.1671547563353151No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGT9630.16032871548895264No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9540.15883031627877553No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG9330.15533405145502888No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9220.15350267464259015No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCC9110.15167129783015146No Hit
CAGTAATAATCAGATTCATCATGTGCCTGGGCCCCCGTGATGGTGAGGGC9040.1505058762222359No Hit
GTACATGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTCATGAGTGTCCC9020.15017289861997432No Hit
CACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGAGGGCGGGAACA8770.14601067859170452No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAGCACTCGCCGC8750.14567770098944294No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC8750.14567770098944294No Hit
CATCTACAGCACAAGCACTCGCCGCTCTGGGGTCCCTGATCGCTTCTCTG8640.14384632417700424No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTGCTTACTACCCCAGCTGG8450.1406830369555192No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8390.13968410414873444No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA8380.13951761534760365No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTTAATTCT8120.13518890651820306No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7970.13269157450124117No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT7950.13235859689897958No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGA7870.13102668648993326No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7820.1301942424842793No Hit
GGTATCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACA7790.12969477608088692No Hit
ATATGGGTAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTC7680.1278633992684482No Hit
CTCTACTGCTTACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGGCTC7590.1263650000582711No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG7470.12436713444470159No Hit
ATATAGAGCACACAGTAATAATCAGATTCATCATGTGCCTGGGCCCCCGT7300.12153682482547812No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTAC7240.12053789201869336No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC7090.11804056000173148No Hit
GCTCTATATGGGTAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGA7060.1175410935983391No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT7060.1175410935983391No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC6960.11587620558703118No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA6930.11537673918363882No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6870.11437780637685406No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6780.11287940716667694No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG6670.11104803035423823No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6650.11071505275197664No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6480.10788474313275317No Hit
GTCTCTACTGCTTACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGGC6390.10638634392257605No Hit
ACCCATATAGAGCACACAGTAATAATCAGATTCATCATGTGCCTGGGCCC6340.10555389991692209No Hit
GATCAGGAGTTAATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCA6290.10472145591126814No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGG6140.10222412389430625No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTG6140.10222412389430625No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTGCTTACTA6100.1015581686897831No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGATACCAGCTGG6080.1012251910875215No Hit
CTACTGCTTACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGGCTCCA6070.1010587022863907No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6020.10022625828073675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12950.061.1314471
CGTTAGT350.004084156460.0494772
GGCGGGG5100.058.003834145
TCAACGC14600.054.222764
ATCAACG14900.053.1310233
TATCAAC15250.051.9116252
CAACGCA15250.051.9116255
GAATACG2350.050.6800549
AACGCAG15800.050.1045726
TACATGG26300.045.284462
GTACATG26650.044.952611
ACATGGG27300.043.625693
TTCGGAT3001.8189894E-1243.386868145
GTGCTAG2950.042.7470861
CGCAGAG18300.042.494038
CTTAGAC700.001250211740.0329863
CATGGGA11750.039.351574
ACGCAGA19900.039.0774277
ATGGGAG8500.037.9135935
AGCACGT2204.9006485E-737.64976145
Produced by FastQC (version 0.11.5)