FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360813
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATACA47500.34905604223357656No Hit
GTATAATAACTGGCCTCGGACGTTCGGCCAAGGAACCAAAGTGGAAATCA39580.2908555400337886No Hit
GTATATTACTGTGCGAAATATGATAGTTATGGCATTGGCTGGAAGACACC37820.2779220951005024No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26440.19429561592959504No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT25420.18680009670689507No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA24110.1771734984895059No Hit
TTATTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT21190.15571573757746288No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21150.15542179564716094No Hit
GTTATTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC20940.15387860051307564No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT20210.1485141602850649No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATACAGTG19210.1411656120275159No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18300.13447843311314633No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17840.1310981009146738No Hit
GCCGTATATTACTGTGCGAAATATGATAGTTATGGCATTGGCTGGAAGAC17360.1275707977510503No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT15110.11103656417156507No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC15040.11052216579353666No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGCT14680.10787668842081903No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA14640.10758274649051706No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14520.1067009206996112No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG14380.10567212394355432No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG14300.1050842400829504No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCTGGGGAAAGAGCCACCCTCTCCT14070.10339407398371415No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA13870.10192436433220436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24950.035.5109521
ATCAACG24900.033.2484053
TCAACGC25600.032.3392684
TATACGG26450.031.300015
ATACGGC26600.030.5822286
TATCAAC28650.029.9016172
TACGGCC27600.029.7350087
CAACGCA27400.029.6893185
AACGCAG28000.029.5673316
GTAATAT29550.029.4954871
CGCAGAG27750.029.3148568
GTACACG14000.029.3102255
CAGCGCT7650.029.1724289
TACATGG39550.029.123652
GTACATG39950.029.0293181
GGCAGCG8950.028.1525237
AATATAC31200.028.1499443
GTATGTA15450.027.507511
ACATGGG40100.027.4675163
CGTATAC1050.007979576527.424773
Produced by FastQC (version 0.11.5)