FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423332
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.23055190725010158No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT7420.17527614260202395No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA6690.15803199380155528No Hit
CCCCTATATTGGTAATAGATAGAGATACTGAGAGGCCCTCAGGGATCCCT6380.15070913609176723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.15023669365887765No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6340.14976425122598813No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA6300.148819366360209No Hit
CTTATGACCTGGCACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG6220.1469295966286508No Hit
CCCTGAGACCTGACGATACGGCTACATATTTTTGTGCGAGAGGGGAGCAT6070.14338627838197915No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6040.14267761473264481No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT5990.14149650865042093No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5720.135118535806412No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC5530.13063033269396124No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA5240.12377991741706273No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5090.12023659917039108No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5080.12000037795394632No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC5070.11976415673750154No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA5040.11905549308816721No Hit
GACTATTACTGTCAATCAGGAGACAGCAGTGGTTATTATGTCTTCGGCAC4860.11480351119216123No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC4830.11409484754282692No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT4800.11338618389349257No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG4790.1131499626770478No Hit
CCCCAGGACAGACGGCCACGATCACCTGCTCTGGAGACGGATTGCCGAAC4570.10795309591526273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.10653576861659407No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT4480.10582710496725974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.085.165721
GTATCAA11700.077.7878951
TATCAAC15700.057.5234072
TCAACGC15750.056.4517864
ATCAACG16100.055.224573
CAACGCA16400.054.2143675
AACGCAG16900.052.196146
CGCAGAG21650.040.7443288
ACGCAGA21700.040.6504487
CAGCGCT2007.2759576E-1238.5050054
GCAGAGT24050.036.6783649
GTACATG15300.035.233341
TACATGG15700.034.7815932
CATGGGG11800.032.0380674
ACATGGG16900.031.897643
GTCTTAT950.00560491329.4775181
AGCGCTG2652.0736479E-1029.060385
TACTACA1454.4246422E-528.9692842
GCAGCGC2901.8189894E-1128.9692848
TACTGGT4200.028.3370192
Produced by FastQC (version 0.11.5)