FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593334
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12610.21252785109230213No Hit
ATATTACGCAGACTCATTGAAGGGCCGATTCACCATCTCCAGAGACAACG10860.1830335022095481No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10520.17730317156947015No Hit
GTTTATTACTGTGGCAGAGCGGGCTTTGGGCACAGTTGGGCAGTTGACCT10440.1759548584776871No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA10130.17073014524702781No Hit
GACCTAGGACGGTCAACCTGGTCCCTCCGCCGAACACCCACTGATTGTTC9130.15387623159973976No Hit
GCCTTGGGCTGACCTAGGACGGTCAACCTGGTCCCTCCGCCGAACACCCA8160.13752793536187038No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8100.13651670054303308No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7500.12640435235466027No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7430.1252245783993501No Hit
GTAATAAACAGCCGTGTCCTCGGCTGTCAGGCTATTCATCTGCAGATACA7280.12269649135225691No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7110.11983132603221795No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAG6850.11544930848392307No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6800.11460661280155865No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6740.11359537798272137No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6610.11140436920857393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.11073021266268239No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6500.10955043870737224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.10702235166027904No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG6300.10617965597791462No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT5960.10044932533783671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15900.081.578031
GGTATCA5300.071.43591
ATCAACG19150.067.3670653
TCAACGC19500.066.517474
CAACGCA19700.065.842165
TATCAAC19700.065.486262
AACGCAG20400.063.582886
CGCAGAG24550.052.834658
ACGCAGA31500.041.1774837
AACGTGG7300.039.087517145
TACATGG22000.037.2873732
GCAGAGT35250.036.79699
GTACATG22350.036.3897481
GCTATAC800.0023994335.0565033
TAGATAG900.004272932731.1613395
GCACTAG1153.8458884E-430.4839151
ACATGGG26900.030.2346063
CATGGGG16200.026.8333744
GCTAGAC1050.00907290526.7097173
GCGGTCA9050.026.274298145
Produced by FastQC (version 0.11.5)