FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593334
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTACTGTGGCAGAGCGGGCTTTGGGCACAGTTGGGCAGTTGACCT10510.17713463243299726No Hit
ATATTACGCAGACTCATTGAAGGGCCGATTCACCATCTCCAGAGACAACG10480.17662901502357864No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA9760.16449419719753122No Hit
GCCTTGGGCTGACCTAGGACGGTCAACCTGGTCCCTCCGCCGAACACCCA9340.15741555346567027No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8910.15016837059733643No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8330.14039310068190936No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8230.13870770931718054No Hit
GACCTAGGACGGTCAACCTGGTCCCTCCGCCGAACACCCACTGATTGTTC8090.13634816140656023No Hit
GTAATAAACAGCCGTGTCCTCGGCTGTCAGGCTATTCATCTGCAGATACA7550.1272470480370247No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7450.12556165667229588No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAG7340.12370772617109418No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6640.11190998661799256No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6330.10668527338733326No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6010.1012920210202011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.072.8344651
TCAACGC10600.062.184144
CAACGCA10950.060.836915
ATCAACG10850.060.7513283
TATCAAC11000.059.9228942
AACGCAG11400.058.4354486
GGTATCA4150.054.0703931
CGCAGAG14450.044.1602178
GTACATG20200.040.268391
TACATGG21100.038.5507852
ACATGGG23600.034.1698843
AACGTGG8800.031.68548145
ACGCAGA20700.031.1655757
CATGGGG16100.030.0525174
GTTCTAT1754.888485E-628.0490171
GTACATA1754.888485E-628.0490171
TACTGGT3002.910383E-1128.0490172
GTACTGG4250.028.0490171
CAAAGCG1750.00757887827.710035145
GCAGAGT23800.026.2222969
Produced by FastQC (version 0.11.5)