FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594083
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT49340.3095196423272816No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37810.2371896570002942No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31840.19973865852656353No Hit
GATGCAGACAGTGTGCAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGG30860.19359092343372333No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTACTATTGTCAAC30160.18919968408169463No Hit
ATTAATAACTGGTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCCAA30090.18876056014649176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29750.18662767246122064No Hit
GTGTGCAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATTTGGGACA26680.16736895130303756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26440.16586338352519914No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG26350.1652987956085097No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25030.1570181728303984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22190.13920228745931046No Hit
GTCTGCATCAACGATCAGGAGTTTGGGGGCTTTCCCTGGTTTCTGCTGAT21740.13637934787586345No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19980.12533851750504835No Hit
GTATTAATAACTGGTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCC19820.12433480565315608No Hit
CTTTGCACACTGTCTGCATCAACGATCAGGAGTTTGGGGGCTTTCCCTGG19280.12094727815301963No Hit
GGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG18940.11881439046774855No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18610.11674423477322073No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18290.11473681106943616No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGGGGGAGAAGTCGCGACCCTGT18180.11404675917126021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.11316851130085447No Hit
ATTGTCAACAGGGTCGCGACTTCTCCCCCACTTTCGGCGGAGGGACCAGG17590.11034557171740744No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17400.10915366389328536No Hit
GATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTA17220.10802448805990654No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17210.10796175606916328No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16720.10488788852274318No Hit
TGCAGACAGTGTGCAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGAT16600.10413510463382397No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCCTGGTCC16420.10300592880044515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31450.056.926251
TCAACGC40900.043.8285564
CAACGCA42700.041.980985
TATCAAC43100.041.3026472
AACGCAG44300.040.302236
CGCAGAG48150.037.0797278
ACGCAGA54350.032.849847
GCAGAGT56800.031.4329039
ATCAACG60150.029.9216483
TACATGG48850.029.3593652
GTACATG49950.029.1942581
ACATGGG49800.028.9122413
TAAACGG5000.027.3567665
CATGGGG25300.023.0486564
ATAAACG6850.022.0703814
AACGGCC6550.020.8830267
AAACGGC6600.020.7248216
GTAAGAC4551.6916601E-1020.5689953
AGTTACC42100.020.3491389
GAGTTAC41400.020.3454198
Produced by FastQC (version 0.11.5)