FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594083
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT43010.26981029218679325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29820.1870667963964235No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25700.16122121621019733No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25400.15933925648789932No Hit
ATTAATAACTGGTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCCAA24490.15363064533026197No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTACTATTGTCAAC23550.14773383820039485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21020.13186264454234817No Hit
GATGCAGACAGTGTGCAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGG20930.13129805662565877No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20850.13079620069971262No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20060.1258403734309945No Hit
GTCTGCATCAACGATCAGGAGTTTGGGGGCTTTCCCTGGTTTCTGCTGAT19500.12232738194937152No Hit
GTGTGCAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATTTGGGACA19240.12069635019004657No Hit
GTATTAATAACTGGTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCC18940.11881439046774855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18290.11473681106943616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16860.10576613639314893No Hit
CTTTGCACACTGTCTGCATCAACGATCAGGAGTTTGGGGGCTTTCCCTGG16230.10181402097632307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22900.041.5258981
TCAACGC26750.034.447154
TATCAAC29750.031.941412
CGCAGAG30400.029.8376128
CAACGCA31450.029.7570465
ACATGGG46000.029.4216583
TACATGG46700.029.2889542
TACACGG4200.029.138476
GTACATG48300.029.0849321
AACGCAG32100.028.9302226
AAATCCG4100.026.3374985
ACGCAGA36650.025.3386157
GTACACG4550.025.314875
TCCGCTA4051.8189894E-1224.8851418
AATCCGC4450.024.266016
GCAGAGT38400.023.996399
CCGTGCA8450.022.150519
TAAACGG4609.094947E-1221.9097465
ATACGAG1650.002710539621.8148983
CATGGGG26050.021.2789484
Produced by FastQC (version 0.11.5)