FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130943
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTTCAGATCTCAGGCTCCTCAGGTCCATGAAGG94890.30307163049598795No Hit
GTATATTACTGTGCGCGATATTGTAGTAGCACCAACTGCTCCTTCCACTA81160.25921902762202953No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG74880.23916117284792476No Hit
ATATTACTGTGCGCGATATTGTAGTAGCACCAACTGCTCCTTCCACTACG73090.233444045452121No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGTTTGCCCTGGTGTTCGG71070.22699231509484522No Hit
ATATTGTAGTAGCACCAACTGCTCCTTCCACTACGGTTTGGACGTCTGGG64000.20441125884438008No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC57890.1848963714765807No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA49120.15688564116306175No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47450.1515517848775912No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA47140.15056166784256372No Hit
CAGTAATATACGGCCGTGTCTTCAGATCTCAGGCTCCTCAGGTCCATGAA47080.15037003228739712No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC45940.1467289567392316No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC44110.1408840723066501No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCGTGCA42520.13580573009473504No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC41420.13229241158334723No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG41040.13107871973395874No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40410.12906654640470938No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAG38720.12366881160084996No Hit
GCCGTATATTACTGTGCGCGATATTGTAGTAGCACCAACTGCTCCTTCCA37010.11820719827860168No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG36300.11593951087579685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33360.10654936867263315No Hit
ATATACGGCCGTGTCTTCAGATCTCAGGCTCCTCAGGTCCATGAAGGCTG33130.10581476571116114No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCT32820.10482464867613368No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT32320.10322768571641196No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCC32110.10255696127332885No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT31740.10137520868313477No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA31350.10012957757455183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50650.078.324421
GGTATCA22500.062.1918031
ATCAACG62500.062.132033
TCAACGC63050.061.4757734
CAACGCA63000.061.17665
AACGCAG67750.057.1562166
TATCAAC71150.055.0846752
CGCAGAG78750.049.1717038
ACGCAGA88100.043.871447
GCAGAGT92150.041.8651859
GTACATG85100.030.9369281
TACATGG84650.030.384222
TACACGG29800.029.7084056
GTACACG29900.029.6350735
ATACGGC49000.028.2030226
TATACGG49400.027.999255
CAGCGCT18000.027.5905369
ATATACG52100.027.2417724
ACACGGG32600.027.1563267
TACGGCC50950.026.9819157
Produced by FastQC (version 0.11.5)